姜黄素诱导胃癌细胞SGC-7901凋亡机制  

Mechanism of curcumin-induced apoptosis of gastric cancer SGC-7901 cells

作　　者：黄果[1] 王佑权[1] 刘国文[1] 陈娟[1] 

机构地区：[1]南华大学附属第二医院乳腺甲状腺外科放疗科,湖南衡阳421001

出　　处：《中国公共卫生》2017年第7期1082-1085,共4页Chinese Journal of Public Health

基　　金：衡阳市基础研究计划(2009KJ45)

摘　　要：目的探讨姜黄素诱导人胃癌细胞SGC-7901凋亡的可能机制。方法体外培养人胃癌细胞SGC-7901,细胞计数盒8(CCK8)法观察不同浓度姜黄素对胃癌细胞SGC-7901的增殖抑制作用;流式细胞仪检测SGC-7901细胞周期;Western blot检测SGC-7901细胞核转录因子κB(NF-κB)、livin、caspase-3蛋白表达情况。结果与对照组比较,各剂量姜黄素组SGC-7901细胞增殖活力均受到抑制,呈剂量效应关系(r=0.901,P<0.01)与时间效应关系(r=0.389,P<0.01);与对照组(2.8%)比较,20、40、60μmol/L姜黄素组SGC-7901细胞凋亡率[分别为7.7%、13.4%、20.7%]明显升高(P<0.05);与对照组(58.59%)比较,20、40、60μmol/L姜黄素组SGC-7901细胞周期G1期比例[分别为60.24%、65.13%、68.35%]明显升高(P<0.05);与对照组比较,姜黄素组SGC-7901细胞内NF-κB、livin蛋白表达降低,caspase-3蛋白表达升高(P<0.05),呈剂量效应关系。结论姜黄素可抑制人胃癌细胞SGC-7901的活性,其机制可能与姜黄素下调NF-κB信号通路,促进livin解除对caspase的抑制效应而激活caspase-3,发挥其诱导细胞凋亡作用有关。Objective To explore possible mechanism of curcumin-induced apoptosis of human gastric cancer SGC-7901 cells.Methods Human gastric cancer SGC-7901 cells were cultured in vitro.Cell counting kit-8 （CCK-8） was used to evaluate anti-proliferative effect of curcumin at different concentrations on SGC-7901 cells;flow cytometry was adopted to assess cell cycle of the SGC-7901 cells.The expressions of nuclear factor-kappa B （NF-κB）,livin and caspase-3 in SGC-7901 cells were determined with Western blot.Results Compared with that of the control group,the proliferation of SGC-7901 cells treated with different doses of curcumin was inhibited in dose-dependent （r=0.901,P〈0.01） and time-dependent manner （r=0.389,P〈0.01）.Compared with that （2.8%） of the control group,the apoptosis rate of SGC-7901 cells treated with 20,40,and 60 μmol/L curcumin were significantly higher （7.7%,13.4%,and 7.7%;P〈0.05 for all）.Compared with that （58.59%） of the control group,the proportion of cells arrested in G1 phase for the SGC-7901 cells treated with 20,40,and 60 μmol/L curcumin were significantly higher （60.24%,65.13%,and 68.35%;P〈0.05 for all）.Compared with those of the control group,significantly decreased expression of NF-κB and livin and increased expression of caspase-3 protein were observed in SGC-7901 cells treated with curcumin in a dose-effect manner （P〈0.05 for all）.Conclusion Curcumin inhibits the activity of gastric cancer SGC-7901 cells and the mechanism of the effect may be related to the induction of cell apoptosis due to decreased NF-κB and the promotion of livin＇s inhibition effect on caspase and the activation of caspase-3. Key words： curcumin gastric cancer cell cell apoptosis nuclear factor-kappa B livin caspase-3

关 键 词：姜黄素 胃癌细胞 细胞凋亡 核转录因子κB(NF—κB) LIVIN caspase-3 

分 类 号：R114[医药卫生—卫生毒理学]