津田芜菁BrPIP1的克隆及其在非生物胁迫下的表达分析  

Cloning and Expression of the BrPIP1 Gene in Brassica rapa subsp. rapa ‘Tsuda'Under Abiotic Stress

作　　者：宋珊[1] 马璇[1] 闫海芳[1] SONG Shan MA Xuan and YAN Haifang(College of Life Sciences, Northeast Forestry University, Harbin 150040, China)

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040

出　　处：《园艺学报》2017年第7期1335-1343,共9页Acta Horticulturae Sinica

基　　金：中央高校基本科研业务费专项(DL12CA10);黑龙江省哈尔滨市青年后备人才项目(2015RQQXJ062);国家自然科学基金项目(31272200;30730078)

摘　　要：克隆得到津田芜菁(Brassica rapa ssp.rapifera‘Tsuda’)质膜内在蛋白(plasma membrane intrinsic proteins,PIPs)基因全长cDNA序列,命名为BrPIP1(GenBank登录号为KJ173685),全长为1 056 bp,开放阅读框为861 bp,编码286个氨基酸。荧光定量PCR分析BrPIP1在不同组织以及其在温度、脱水、渗透、ABA和盐等非生物胁迫条件下幼苗中的表达表明,该基因表达具有组织特异性,在花瓣中表达量最高,花蕾中次之;在上述非生物胁迫下表达量都有不同程度增加,暗示BrPIP1在非生物胁迫应答中发挥作用。In this study, we cloned the plasma membrane intrinsic proteinsl （PIP1） gene from Brassica rapa ＇Tsuda＇ , which was designated as BrPIPI with the accession number of KJ173685 in the NCBI GenBank. The full cDNA sequence of the BrPIP1 gene has 1 056 base pairs, containing an open reading frame of 861 bp encoding a protein of 286 amino acids. We determined the expression of the BrPIP1 gene in different tissues and under different abiotic stress conditions, such as extreme temperature, dehydration, adverse osmosis, abscisic acid （ABA） and salt stress by quantitative-PCR analysis. The results demonstrated that the highest expression levels of the BrPIP1 gene could be reached in the petal, followed by the bud, with tissue specificity. The expression of the BrPIP1 gene was upregulated under the aforementioned stress conditions, suggesting that the BrPIP1 gene may play a role in response of abiotic stresses.

关 键 词：芜菁(蔓菁) PIP1 基因克隆 非生物胁迫 表达分析 

分 类 号：S631.3[农业科学—蔬菜学]