PHP14沉默对肺癌细胞凋亡的影响及其机制  

The Effect of PHP14 Knockdown on Lung Cancer Cells Apoptosis and Its Mechanism

作　　者：徐安健[1] 李艳萌[1] 李斯文[1] 乌姗娜[2] 张蓓[1] 黄坚[1] XU An-jian LI Yan-meng LI Si-wen WU Shan-na ZHANG Bei HUANG Jian(Experimental Center, Beijing Friendship Hospital, Beijing 100875, China Clinical Laboratory Center, Beijing Friendship Hospital, Beijing 100875, China)

机构地区：[1]首都医科大学附属北京友谊医院科研实验中心,北京100875 [2]首都医科大学附属北京友谊医院临床检验中心,北京100875

出　　处：《中国生物工程杂志》2017年第7期12-17,共6页China Biotechnology

基　　金：北京优秀人才培养项目(2016000021469G227);北京友谊医院院启动项目(yyqdkt201516)资助项目

摘　　要：目的:在肺癌细胞中沉默PHP14,并研究其对肺癌细胞凋亡的影响及其可能机制。方法:利用shRNA稳定沉默肺癌细胞株A549中PHP14的表达,并利用Annexin V、PI双染和流式细胞仪检测细胞在正常培养条件下和凋亡诱导条件下的凋亡情况;通过皮下接种裸鼠,探讨PHP14沉默对肺癌细胞体内成瘤的影响,并利用Realtime-PCR和Western blotting检测PHP14沉默后凋亡相关基因的表达情况。结果:成功获得了PHP14稳定沉默的肺癌细胞株,发现PHP14沉默可促进肺癌细胞诱导性凋亡,并抑制肺癌细胞的体内成瘤能力,并且发现PHP14沉默对肺癌细胞凋亡的影响可能是通过抑制Bcl-2表达实现的。结论:PHP14沉默可促进肺癌细胞诱导性凋亡,并可能通过抑制Bcl-2影响肺癌细胞凋亡。Objective： To knockdown PHP14 expression in lung cancer cells, and investigate the effect of PHP14 knockdownon lung cancer cells apoptosis and its mechanism. Methods： Using shRNA stably knockdown PHP14 expression in lung cancer cell A549; detected apoptosis of control cells and PHP14 RNAi cells under normal culture and apoptosis-induced treatment using double staining of Annexin V and PI by flow cytometry; investigated the tumor growth of control cells and PHP14 RNAi cells in vivo, and detected the expression of apoptosis related genes by Realtime-PCR and Western blotting. Results： The expression of PHP14 was stably knockdown successfully, and knockdown of PHP14 could promote induced-apoptosis, as well as the tumor growth in vivo; furthermore, the effect of PHP14 knockdown on lung cancer cells apoptosis possobly via Bcl-2 suppression was found. Conclusion： knockdown of PHP14 could promote induced-apoptosis possobly via Bcl-2 suppression.

关 键 词：PHP14 细胞凋亡 体内成瘤 BCL-2 

分 类 号：Q819[生物学—生物工程]