高原地区55型腺病毒的病原分离及全基因序列分析  

Pathogen isolation and whole-genome sequence analysis of human adenovirus type 55 outbreak on plateaus

作　　者：王文博[1] 刘媛[2] 周奕帆[1] 古良琪[1] 张雪莲[1] 张林[1] 陈锚锚[1] 杨显君[1] 邹自英[2] 郭平[1] 邱薇[1] 胡小兵[1] 范泉水[1] 

机构地区：[1]成都军区疾病预防控制中心,成都610021 [2]成都军区总医院检验科,成都610083

出　　处：《军事医学》2017年第6期453-456,共4页Military Medical Sciences

基　　金：全军后勤"十二五"重大专项资助项目(AWS11L009);军队"十二五"重点课题资助项目(BWS14J025);国家自然科学基金资助项目(81301445);全军医学科技青年培育项目(14QNP057);上海市医学生物防护重点实验室开放课题资助项目(SKLM1401)

摘　　要：目的探讨高原地区55型腺病毒(HAdV-B55)基因变异特点。方法采集驻西藏某部队55型腺病毒感染者咽拭子标本,采用HEp-2分离病毒株,通过分片段PCR扩增和测序获得HAdV-B55毒株的全基因序列,通过与内地毒株进行序列比对,发现其变异特点。结果从55型腺病毒感染者咽拭子标本分离得到HAdV-B55毒株,命名为LS89/Tibet/2016,通过PCR产物测序拼接,获得了本次流行的HAdV-B55毒株的全基因序列,Gen Bank登录号为KY002683。通过MEGA6软件比对,发现该病毒基因序列并未发生大片段重组,与内地以往流行毒株(QS-DLL株)序列相似性为99.9%。结论本次分离出的病毒基因序列未发生大片段重组。本研究为揭示55型腺病毒的进化特点及今后高原地区制订疾病防控策略提供了依据。Objective To investigate the variation characteristics of adenovirus type 55（ HAdV-B55） gene on plateaus. Methods Throat swabs were collected from HAdV-B55 infected patients and used for virus isolation in HEp-2cells. The whole-genome sequence was obtained by PCR and sequencing. HAdV-B55 gene sequence was blast with the previously reported virus. Results HAdV-B55 strains were isolated from throat swabs,which were named LS89/Tibet/2016. The whole-genome sequence was obtained and submitted to Gen Bank with the accession number of KY002683. No large fragment gene recombination was found between this HAdV-B55 strain and previous strains,and the sequence similarity with QS-DLL strain was 99. 9%. Conclusion This study provides more information for the evolution patterns of adenovirus 55 and will contribute to the prevention and control of HAdV-B55 infection in the future.

关 键 词：高原 腺病毒 HAdV-B55 病原分离 全基因测序 

分 类 号：R824[医药卫生—临床医学]