氢气通过诱导血红素加氧酶1表达减轻氧中毒急性肺损伤的实验研究  

Hydrogen reduces hyperoxic acute lung injury by inducing heme oxygenase 1 expression

作　　者：韩文杰[1,2] 马玉媛[1] 贾俊婷[1] 欧敏[2] 章金刚[1] 

机构地区：[1]军事医学科学院野战输血研究所,北京100850 [2]海军总医院,北京100048

出　　处：《军事医学》2017年第6期498-501,共4页Military Medical Sciences

基　　金：海军总医院创新培育基金资助项目(CXPY201409)

摘　　要：目的研究氢气(H_2)对于防治氧中毒急性肺损伤(HALI)的作用机制。方法将30只雄性SpragueDawley大鼠随机分为3组:正常对照组、氧中毒模型组(HALI组)和H_2治疗组(H_2组)。其中正常对照组大鼠仅暴露于常压空气,HALI组和H_2组大鼠暴露于100%医用纯氧,每隔12 h分别腹腔注射医用生理盐水和氢饱和生理盐水(10 ml/kg)。60 h后对各组大鼠进行动脉血氧分压检测和肺组织病理学检查,并应用RT-q PCR和Western印迹技术检测肺组织中血红素加氧酶1(human heme oxygenase 1,HO-1)的mRNA转录水平和蛋白表达水平。结果与HALI组相比,H_2组大鼠因慢性纯氧暴露导致的肺损伤程度明显减轻,动脉血氧饱和度升高,HO-1蛋白及其mRNA在肺组织中的表达显著升高。结论 HO-1可能在H_2防治大鼠HALI中发挥重要作用。Objective To investigate the mechanism by which hydrogen（ H_2） helps prevent acute lung injury induced by hyperoxia（ HALI） in rats. Methods Thirty male Sprague-Dawley rats were randomly divided into three groups： control group,HALI group and H_2 group,with 10 rats in each group. The control group was exposed to air at atmospheric pressure. Rats in HALI and H_2 groups were exposed continuously to pure oxygen（ 100% O2） for 60 hours and during this period,10 ml/kg of normal saline or H_2-saturated normal saline was given every 12 hours by intraperitoneal injection to the HALI and H_2 groups, respectively. After treatment, the arterial partial pressure of oxygen was examined and histopathological examination was conducted in each group. Then,RT-q PCR and Western blotting were performed to measure the transcriptional level and protein expression of heme oxygenase 1（ human heme oxygenase 1,HO-1） in rat lung tissue. Results Compared with the HALI group,H_2 group showed significantly decreased severity of lung injury and a marked increase in the arterial oxygen saturation. Besides,H_2 treatment induced up-regulation of HO-1 mRNA and protein levels. Conclusion The findings suggest that HO-1 may play an important role in the protection against HALI by H_2.

关 键 词：氢 氧 中毒 急性肺损伤 血红素加氧酶1 

分 类 号：R563.8[医药卫生—呼吸系统] R595.1[医药卫生—内科学]