根施外源甜菜碱对胡杨幼苗叶片积累甜菜碱与耐盐特性的影响  

Effect of Root-applied Glycinebetaine on Glycinebetaine Accumulation and Salinity Tolerance of Seedling of Populus euphratica

作　　者：郭振洁[1,2] 王芸[1,2,3] 何学敏[1,2,4] 张雪妮[1,2,4] 吕光辉[2,4] 

机构地区：[1]新疆大学资源与环境科学学院,新疆乌鲁木齐830046 [2]绿洲生态教育部重点实验室,新疆乌鲁木齐830046 [3]河北省辛集市新城镇政府,河北新城052360 [4]新疆大学干旱生态环境研究所,新疆乌鲁木齐830046

出　　处：《干旱区研究》2017年第4期847-855,共9页Arid Zone Research

基　　金：国家自然科学基金项目(31560131);国家科技支撑计划项目(2014BAC15B04)资助

摘　　要：在不同盐分处理下(0、100、200、300、500 mmol·L^(-1)Na Cl)对土壤进行盆栽实验,研究根施甜菜碱(GB,5 mmol·L^(-1))对1 a生胡杨(Populus euphratica)幼苗叶片甘氨酸甜菜碱(GB)积累特性与不同时间(第7天、第14天)光合作用的影响。结果表明:(1)盐胁迫单独处理下,相比对照(0 mmol·L^(-1)Na Cl,CK)叶片甜菜碱随盐胁迫强度增大积累量显著增多。盐胁迫和外源GB双重处理下,叶片GB含量总体趋势随盐浓度升高表现为先升高后保持平稳。在低盐胁迫处理时(100~300 mmol·L^(-1)Na Cl)外源GB能诱导叶片产生更多GB,较未经GB处理组叶片GB积累量分别提高44.0%、32.5%和35.9%,而高盐胁迫(500 mmol·L^(-1)Na Cl)则转为抑制,叶片GB含量降低了5.1%。(2)随着盐浓度升高,外源GB虽不能持续促进叶片GB含量,但其对光合维持能力却没有丧失。随着胁迫强度增加,外源GB对叶绿素含量、水分利用效率(WUE)、叶片相对含水量(RWC)及相关光合参数的维持作用越明显。(3)不同时间下盐分和外源GB对胡杨光合作用的影响会改变,外源GB先维持胡杨水分传递过程,后维持CO_2同化能力,最终提高其光合作用。总之,根施GB能够促使胡杨叶片GB积累量发生变化并发挥作用,调节盐胁迫下胡杨光合相关生理过程从而提高其耐盐能力。A pot experiment was carried out so as to investigate the effects of exogenous glycinebetaine on glycinebetaine accumulation and photosynthesis of seedling leaves of Populus euphratica in different time periods（ 7 and 14 days） under different salt stress levels（ 0,100,200,300 and 500 mmol·L^（-1）NaCl）. The results are as follows：（1） Glycinebetaine accumulation of seedling leaves of P. euphratica was significantly increased with the increase of salt stress level. Under the exogenous GB treatment,the glycinebetaine accumulation of seedling leaves of P. euphratica was holistically increased at first and then stabilized with the increase of salt stress level. Under low salt stress（ 100-300 mmol·L^（-1）NaCl）,exogenous GB could be absorbed by roots and help leaves produce more GB,the GB accumulations of leaves were increased by 44. 0%,32. 5% and 35. 9% respectively compared to the control group. Under high salt stress（ 500 mmol·L^（-1）NaCl）,however,the GB accumulation of leaves was restricted by exogenous GB,and it was decreased by 5. 1%;（2） Although the exogenous GB could not be used to continuously promote the GB accumulation in leaves,but the capability of maintaining photosynthesis did not lose. With the increase of salt stress intensity,the effects of exogenous GB on the chlorophyll content,water use efficiency,relative water content and related photosynthetic parameters were more significant;（3） The effects of salt stress and exogenous GB on the photosynthesis of P. euphratica were changed with the time. The exogenous GB maintained the moisture transfer process of P. euphratica,then the CO_2 assimilation capacity,and the photosynthesis was increased. In short,the root-applied GB could change the GB accumulation in leaves of P. euphratica and adjust the photosynthesis process under salt stress,and the salt tolerance of P. euphratica was improved.

关 键 词：盐胁迫 甜菜碱 胡杨幼苗 光合作用 叶绿素 

分 类 号：S792.11[农业科学—林木遗传育种]