白细胞介素13在嗜酸粒细胞性慢性鼻-鼻窦炎合并鼻息肉组织中的表达及其与黏蛋白高分泌的相关性研究  

Study on expression of IL-13 and correlation between IL-13 and MUC5AC in eosinophilic chronic rhinosinusitis with nasal polyps

作　　者：张宇[1] 王向东[2] 王鸿[2] 范尔钟[2] 李颖[2] 宋西成[1] 张罗[2] 

机构地区：[1]青岛大学附属烟台毓璜顶医院耳鼻咽喉头颈外科,山东烟台264000 [2]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科、北京市耳鼻咽喉科研究所、耳鼻咽喉头颈科学教育部重点实验室(首都医科大学)、鼻病研究北京市重点实验室,北京100005

出　　处：《中国耳鼻咽喉头颈外科》2017年第7期371-374,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：长江学者和创新团队(IRT13082);国家自然科学基金重点国际合作研究项目(81420108009);国家自然科学基金(81100704、81441029、81441031、81400444、81570894、81670907);科技部“十二五”国家科技支撑计划课题(2014BAI07B04);首都医学发展基金(2011-1017-06);北京市卫生系统高层次人才计划(2009-2-007、2011-3-039、2011-3-043、2014-3-018);北京市医管局使命计划(SML20150203);北京市自然科学基金(7172054);交叉学科合作项目(xxjc201712)联合资助

摘　　要：目的明确白细胞介素13(IL-13)在嗜酸粒细胞性慢性鼻-鼻窦炎(eosinophilic chronic rhinosinusitis with nasal polyps,EOS-CRSwNP)中的表达,探讨在EOSCRSwNP中IL-13和黏蛋白5AC(mucin 5AC,MUC5AC)的相关性。方法利用免疫组化和ELISA方法观察和测量MUC5AC在对照组鼻黏膜组织和EOS-CRSwNP组织的表达,ELISA法检测IL-13在对照组鼻黏膜组织和EOS-CRSwNP组织的表达;双变量相关性分析研究EOSCRSwNP中IL-13和MUC5AC的相关性。IL-13与原代培养鼻黏膜上皮细胞共孵育,ELISA检测细胞上清中MUC5AC的表达。结果免疫组化染色见MUC5AC主要表达在鼻黏膜上皮,通过ELISA检测,EOS-CRSwNP中MUC5AC和IL-13均较对照组显著升高。双变量相关性分析表明在EOS-CRSwNP中MUC5AC与IL-13存在高度相关,进一步通过IL-13与气液界面原代培养人鼻黏膜上皮细胞共孵育,MUC5AC分泌显著增加。结论 MUC5AC和IL-13在EOSCRSwNP中表达升高,MUC5AC的高分泌与IL-13高表达密切相关。OBJECTIVE To explore expression of IL-13 in eosinophilic chronic rhinosinusitis with nasal polyps（EOS-CRSwNP） and further investigate the correlation between IL-13 and MUCSAC in EOS- CRSwNP. METHODS MUCSAC was detected in tissues from normal nasal mucosa and EOS-CRSwNP by immunohistochemistry and ELISA. We also used ELISA to detect the expression of IL-13 in normal nasal mucosa and EOS-CRSwNE Correlation between IL-13 and MUCSAC in EOS-CRSwNP was investigated by correlation analysis. Secretion of MUCSAC in IL-13 incubated primary air- liquid interface（ALI）-cultured nasal polyp epithelial cells was examined by ELISA. RESULTS MUCSAC is mainly expressed in the epithelium of nasal mucosa by immunohistochemistry. By ELISA, expressions of MUCSAC and IL-13 are higher in EOS-CRSwNP than those in normal nasal mucosa. Correlation analysis shows that there exists a high correlation between MUCSAC and IL-13 in EOS-CRSwNP. IL-13 upregulates expression of MUCSAC in IL-13 incubated primary ALI-cultured nasal polyp epithelial cells for 7 or 14 days. CONCLUSION Expressions of MUCSAC and IL-13 are higher in EOS-CRSwNP than those in normal nasal mucosa respectively, and MUCSAC correlates with IL-13 highly in EOS-CRSwNP.

关 键 词：嗜酸细胞 鼻息肉 白细胞介素13 黏蛋5AC 

分 类 号：R765[医药卫生—耳鼻咽喉科]