嵌合基因gtfB-ctxB在防龋用转基因生菜的遗传稳定性研究  

Genetic stability of anti-caries gene(gtfB-ctxB) in progenies of transgenic lettuce

作　　者：王海慧 初可嘉 管晓燕[3,4] 白国辉[3,4] 钱昱霏[3,4] 周皓琳 范繁[3,4] 刘建国[3,4] 

机构地区：[1]贵阳市口腔医院儿童口腔科,贵州550002 [2]贵阳市口腔医院正畸科,贵州550002 [3]遵义医学院口腔学院 [4]贵州省高等高校口腔疾病研究特色重点实验室遵义市口腔疾病研究重点实验室

出　　处：《环境与健康杂志》2017年第5期401-403,共3页Journal of Environment and Health

基　　金：国家自然科学基金(81260164);贵州省高层次人才科研条件特助经费(201130);贵州省科技创新人才团队资助项目(20134026);贵州省重点学科建设资助项目(20120704)

摘　　要：目的研究防龋用转基因生菜后代植株中外源嵌合基因gtfB-ctxB的遗传稳定性。方法种植T3、T4代外源嵌合基因gtfB-ctxB生菜植株64株、25株,分别在植株幼苗期、开花期、种子成熟期采集叶片、花、茎、种子等不同部位,采用PCR技术检测外源基因gtfB-ctxB,比较T_2~T_4连续世代转基因生菜的阳性率。结果 T_3、T_4代转基因生菜阳性植株分别为40株、15株,PCR阳性率分别为62.5%和60.0%。幼苗期、开花期、种子成熟期植株不同部位(叶片、花、茎、种子)的PCR阳性率检测结果一致。结论嵌合基因gtfB-ctxB能够在防龋用转基因生菜后代植株中稳定遗传。Objective To know genetic stability of anti-caries gene（gtfB-ctxB） in progenies of transgenic lettuce. Methods Genomic DNA was extracted from T_3,T_4 generation of the transgenic lettuce plants in three in different growth stages（young seedling,flowering,seed maturity） and different position（leaves,flowers,stems,seeds）. PCR technology was used to detect the target gene gtfB-ctxB in progenies of transgenic lettuce. Identification and selection transgenic lettuce plants which containing the target gene were conducted. The statistics of the positive rate in different generations were collected to compare whether there was a significant differences among the three generations. Results The transgenic lettuce plants genomic DNA of T_3 and T_4 generation which carrying the target gene gtfB-ctxB was amplified by PCR. The positive transgenic plants were 40,15 respectively in each generation. The positive rates were 62.5% and 60.0% respectively. The experimental results of T_3,T_4 generation of the transgenic lettuce plants in three in different growth stages（young seedling,flowering,seed maturity） and different position（leaves,flowers,stems,seeds） were consistent. Conclusion The transgenic lettuce plants carrying the anticaries gene gtfB-ctxB were obtained in the present study and the results demonstrate that the target gene gtfB-ctxB can be stably inherited to the progeny of transgenic lettuce plants.

关 键 词：转基因生菜 防龋疫苗 变异链球菌 遗传稳定性 

分 类 号：R994.6[医药卫生—毒理学]