BALB/c小鼠LLNA:BrdU-ELISA改良法在15种化学物皮肤致敏性评价中的应用  

Performance of the LLNA:Brdu-ELISA using BALB/c mice for 15 substances in skin-sensitivity evaluation

作　　者：冼静雯 郭煜堂 陈宁 李俊鹏 王平 秦美蓉 王晓炜 

机构地区：[1]深圳市药品检验研究院深圳药品质量标准研究重点实验室,广东深圳518057 [2]南方医科大学

出　　处：《环境与健康杂志》2017年第5期438-440,共3页Journal of Environment and Health

基　　金：深圳市药品检验研究院重点学科团队建设项目

摘　　要：目的探讨BALB/c小鼠Brd U-ELISA改良法在化学物皮肤致敏性评价中的应用。方法采用BALB/c小鼠LLNA:Brd U-ELISA改良法,对13种已知和2种未知致敏性化学物进行评价,并进行方法真实性分析。结果香叶醇(25%)、丁子香酚(25%)、异丁香酚(25%)、反式肉桂醛(20%)、α-己基肉桂醛(25%)、水杨酸己酯(5%)、对苯二酚(5%)、2,4-二硝基氯苯(0.3%)、苯(100%)、氯仿(100%)为致敏阳性,其余5种均为致敏阴性;方法真实性分析显示,该法阳性预测率为90%、阴性预测率为100%、假阳性率为17%、假阴性率为0、灵敏度为100%、特异度为83%、准确度为93%。结论 BALB/c小鼠LLNA:Brd U-ELISA改良法可较好地评估化学物的致敏性,并完善了香叶醇、水杨酸己酯致敏性的相关毒理数据。Objective To validate the LLNA：Brdu-ELISA using BALB/c mice in skin-sensitivity evaluation of chemicals.Methods The LLNA：Brdu-ELISA using BALB/c mice in skin-sensitivity evaluation was carried out for 13 substances with known skin-sensitivity and two substances with unkown skin-sensitivity in order to analyze the authenticity of the method.Results The tests procured 25% geraniol,25% eugenol,25% isoeugenol,20% trans-cinnamaldehyde,25% α-hexyl-cinnamaldehyde,5% hexyl aslicylate,5% hydroquinone,0.3% 2,4-dinitrochlorobenzene,100% benzene,100% chloroform were skin sensitizer and other groups were not. The positive predictive rate,negative predictive rate,false positive rate,false negative rate,sensitivity,specificity,accuracy were 90%,100%,17%,0,100%,83%,93%,respectively. Conclusion The LLNA：BrduELISA using BALB/c mice in skin-sensitivity evaluation is feasible. The study complemented the toxicology data of geraniol and hexyl aslicylate.

关 键 词：局部淋巴结试验 皮肤致敏性 溴脱氧尿嘧啶核苷 酶联免疫吸附测定 

分 类 号：R994.6[医药卫生—毒理学]