新疆北疆亚洲璃眼蜱贝纳柯克斯体的检测  

Detection of Coxiella burnetii in Hya lomma asiaticun from Northern Xinjiang, China

作　　者：刘丹[1] 尹小平[3] 吾热力哈孜·哈孜汗 涂长春[4] 陈创夫[1] 王远志[5] Liu D Chen CF Yin XP Ha zi Wureli Tu CC Wang YZ(Department of Veterinary Medicine, College of Animal Science and Technology, Shihezi University, Shihezi 832000, China A lashankou Entry-Exit Inspection and Quarantine Bureau, Alashankou 833418, China （ Department of Animals Genetics and Breeding, College of Animal Science and Technology, Shihezi University, Shihezi 832000, China Institute of Veterinary Sciences, Academy of Military Medical Sciences, Changchun 130062, China Department of Pathognic Biolgy and Immunology, College of Medicine, Shihezi University, Shihezi 832000, China)

机构地区：[1]石河子大学动物科技学院人兽共患病教研室,石河子832000 [2]石河子大学动物科技学院遗传育种与繁殖教研室,石河子832000 [3]新疆阿拉山口出入境检验检疫局卫生检疫科,阿拉山口833418 [4]中国人民解放军军事医学科学院,长春130062 [5]石河子大学医学院病原生物学与免疫学教研室,石河子832000

出　　处：《中华地方病学杂志》2017年第8期547-551,共5页Chinese Journal of Endemiology

基　　金：基金项目：国家自然科学基金（U1503283、81560338）;新疆生产建设兵团国际科技合作项目（2016AH001）;国家质量监督检验检疫总局科技计划项目（2016IK264）

摘　　要：目的检测新疆北疆亚洲璃眼蜱贝纳柯克斯体感染情况，为减少人和家畜Q热病的发生提供依据。方法2016年4-7月，利用布旗法和动物体表检查法采集北疆福海县、阿拉山口市、石河子市、呼图壁县和克拉玛依市5个县（市）蜱类样本，形态学鉴定蜱种后提取优势蜱的全基因组DNA，采用PCR方法进行蜱种分子学鉴定以及病原体的检测．阳性产物进行16SrRNA测序与BLAST分析，利用Mega6．0软件构建分子遗传进化树。结果新疆北疆5个县（市）共采集蜱822只，其中优势蜱为亚洲璃眼蜱，共采集580只，经16SrRNA测序分析表明，序列均为亚洲璃眼蜱指名亚种，与新疆尉犁县的亚洲璃眼蜱（KF527439）有较高的同源性。应用PCR方法扩增亚洲璃眼蜱贝纳柯克斯体的特异性DNA片段，总阳性率为19．48％（113／580）。BLAST分析表明。扩增的阳性序列与贝纳柯克斯体的同源性最高，达99．66％（296／297）；分子遗传进化树显示，贝纳柯克斯体核酸序列种类较多．具有遗传多样性。结论新疆北疆5个县（市）亚洲璃眼蜱中均存在贝纳柯克斯体感染，提示新疆各县（市）应加强人和家畜Q热的监测。Objective To investigate the infection of CoxieUa burnetii （C. burnetii） in Hyalomma asiaticum （Hy. asiaticum） from Northern Xinjiang, China, and to provide basis for reducing the incidence of Q fever in humans and livestock. Methods Questing ticks were collected via the dragging-flag method and directly sampled from animals in Fuhai County, Alataw Pass, Shihezi City, Hutubi County and Karamay City between April and July in 2016. Genomic DNA was extracted after morphological identification. PCR was used to identify tick species and Coxiella pathogen. BLAST and Mega 6.0 were used in the sequence analysis and the construction of phylogenetic trees. Results A total of 822 ticks were collected from 5 counties （cities） in Northern Xinjiang, among which the dominant ticks were Hy. asiaticum, and 580 were collected. Hy. asiaticum was sequenced, and BLAST results showed that the sequence had a high similarity with that of Yuli County from Xinjiang （accession no. KF527439）. The nucleotide of C. burnetii was amplified via the PCR method, and its positive rate was 19.48% （113/580）. Blast analysis showed that positive sequences had a high similarity with that of C. burnetii （99.66%, 296/297）. Phylogenetic tree showed that the sequences of C. burnetii had genetic diversity. Conclusions C. burnetii extensively exists in Hy. asiaticum from 5 counties （cities） in Northern Xinjiang. These results suggest Q feversurvey should be strengthened in different regions of Xinjiang.

关 键 词：亚洲璃眼蜱 Q热 贝纳柯克斯体 

分 类 号：S852.746[农业科学—基础兽医学]