微波消解-原子荧光光谱法测定发硒  

Determination of selenium in hair by material microwave digestion-atomic fluorescence spectrometry

作　　者：陈宇鸿[1] 陈海红[1] 王益萍[1] Chen Yuhong Chen Haihong Wang Yiping(Physical and Chemical Laboratory, Wenling City Center for Disease Control and Prevention, Wenling 317500, China)

机构地区：[1]浙江省温岭市疾病预防控制中心理化检验科,温岭317500

出　　处：《中华地方病学杂志》2017年第8期598-601,共4页Chinese Journal of Endemiology

摘　　要：目的建立发硒的微波消解一原子荧光光谱测定法。方法发样经微波消解后采用氢化物原子荧光光谱测定头发中的硒含量，并优化测定条件来完善方法。在优化条件下，检测本方法的标准曲线线性关系、样品测定法检测限、精密度、准确度。采用本方法测定人发标准物质硒含量。结果微波消解一原子荧光光谱法检测发硒的检测限为0．095μg／g，标准曲线相关系数为0．9997，相对标准偏差为1．32％～2．48％，平均回收率为93．4％～98．2％。本方法检测人发标准物质硒含量，测定值在标准值不确定度范围内，符合要求。结论成功建立测定发硒的微波消解一原子荧光光谱法，该方法操作简便易行，准确度及精密度均较好，是准确可靠的测定发硒的方法。Objective To determine the hair selenium level by material microwave digestion-atomic fluorescence spectrometry. Methods The selenium in hair was determined by hydride atomic fluorescence spectrometry after microwave digestion, and the method was improved by exploring the test conditions. The linear relationship and linear range of the standard curve, the detection limit, the precision and the accuracy of the sample determination were implemented under the optimized conditions. The selenium value of standard material of human hair was tested by material microwave digestion-atomic fluorescence spectrometry. Results The selenium detection limit of material microwave digestion-atomic fluorescence spectrometry was 0.095 μg/g, the correlation coefficient of standard curve was 0.999 7, the relative standard deviation was 1.32% - 2.48%, the average recovery rate was 93.4% - 98.2%, and the selenium value of standard material of human hair was in the standard value range of uncertainty, which met the requirements. Conclusion The method is simple, accurate and precise, and it is an accurate and reliable method for determination of selenium in hair.

关 键 词：毛发 硒 参考标准 微波消解 原子荧光光谱法 

分 类 号：O657.31[理学—分析化学] R446.1[理学—化学]