食源性吸虫免疫学诊断研究进展  

Progress in immunology diagnosis of food-born trematodes

作　　者：吴玛莉[1] 李安梅[1] 罗维[2] 郭志来[3] Wu ML Li AM Luo W Guo ZL(Institute for Chronic Disease Prevention and Control, Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China Clinical Laboratory, Yichun City Center for Disease Control and Prevention, Yichun 336000, China College of Life Science, Guizhou Normal University, Guiyang 550025, China)

机构地区：[1]贵州省疾病预防控制中心慢性病防治研究所,贵阳550004 [2]江西省宜春市疾病预防控制中心检验科,宜春336000 [3]贵州师范大学生命科学院,贵阳550025

出　　处：《中华地方病学杂志》2017年第8期617-620,共4页Chinese Journal of Endemiology

基　　金：基金项目：贵州省科学技术基金（[201212210）;贵州省优秀科技教育人才省长资金项目（黔省专合字2010-91号）

摘　　要：寄生肝脏、肺及肠道的食源性吸虫可引起多种吸虫病，文中仅介绍了华支睾吸虫、片形吸虫属、后睾吸虫属、并殖吸虫属、棘口吸虫属等常见食源性吸虫当前免疫学诊断技术的研究进展。这些技术主要利用分泌抗原、重组抗原、寄主抗体，通过酶联免疫吸附试验、免疫印迹、免疫荧光、免疫组织化学、蛋白质组学及侧向流动检测系统等进行诊断，综合应用上述方法有利于提高诊断率及精确率。Food-born trematodes which parasitize liver, lung and intestine cause various trematodes diseases. There are a few common trematodes such as Clonorchis sinensis, Fasciola, Opisthorchis, Paragonimus, and Echinostoma. This review provides recent progress in techniques and methods for their immunodiagnosis. These methods are mainly used to diagnose with excretory-secretary antigen, recombinant antigen and antibodies of hosts, by means of enzyme-linked immunosorbent assay, immunoblotting, immunefluorescence, immunehistochemistry, proteomics and a lateral flow detection system so on. It is beneficial to improve diagnostic and accuracy rates when all this methods are used synthetically.

关 键 词：食源性吸虫 分泌抗原 重组抗原 寄主抗体 

分 类 号：R532.2[医药卫生—内科学]