姜黄素对急性游泳训练小鼠骨骼肌损伤的保护作用  

Protective Effects of Curcumin on Intense Swimming Training Skeletal Muscle Damage in Mice

作　　者：高超[1] 刘阳[1] 赵佳[1] 张雪松[1] 王宇飞[1] 杨月欣[1] GAO Chao LIU Yang ZHAO Jia ZHANG Xue-song WANG Yu-fei YANG Yue-xin(National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China)

机构地区：[1]中国疾病预防控制中心营养与健康所,北京100050

出　　处：《中国食物与营养》2017年第6期54-58,共5页Food and Nutrition in China

基　　金：国家科技支撑计划(项目编号:2012BAD33B01);中国疾病预防控制中心营养与健康所青年科学基金(项目编号:NINH201606);中国科协青年人才托举工程(项目编号:YESS20160164)

摘　　要：目的:探讨姜黄素对急性游泳训练小鼠骨骼肌损伤的保护作用。方法:成年雄性BALB/C小鼠随机分为安静对照组、运动对照组、运动+姜黄素组[100 mg/(kg·d)]和安静+姜黄素组[100 mg/(kg·d)]。干预期为4w,干预期最后1w同时进行游泳运动训练,每天训练90min,采用以上运动方式连续运动7d,末次运动完成后即刻处死小鼠。测定血清肌酸激酶(CK)含量及骨骼肌超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、活性氧(ROS)和丙二醛(MDA)水平,并观察骨骼肌普通及超微病理学改变。结果:与运动对照组相比,姜黄素干预明显抑制了游泳运动导致的小鼠血清CK水平的上升(P<0.01),减轻了运动导致的骨骼肌普通及超微病理结构异常。姜黄素干预显著抑制了运动导致的小鼠骨骼肌GSH水平的下降(P<0.05),同时拮抗了ROS和MDA含量水平的上升(P<0.01)。结论:姜黄素对小鼠急性游泳训练导致的骨骼肌损伤具有明显的抗氧化保护作用。[Objective] To investigate protective effect of curcumin against intense swimming training-induced skeletal muscle damage. [Method] Adult male BALB/C mice were randomly divided into 4 groups including rested controls, intense exercise, exercise ＋ curcumin （ 100 mg/kg BW） and rested plus curcumin （ 100 mg/kg BW） supplementation. Curcumin was administrated for 4 weeks and exercise pro- gram （ swimming for 90 min/d） was performed concurrently at the fourth week. Immediately upon final exercise, the mice were sacrificed. Serum creatine kinase and skeletal muscle ultrastructural malformation were examined. SOD, GSH, ROS, and MDA of skeletal muscle were analyzed. [Result] Compared to exercise group, curcumin treatment declined intense swinmming-triggered the leakage of creatine kinase （P 〈 0. 01 ） and skeletal muscle uhrastructural malformation. Cureumin pretreatment evidently alleviated oxidative stress by inhibiting gluta- thione depletion （P 〈 0. 05 ）, ROS over-generation （ P 〈 0. 01 ）, and lipid peroxidation in skeletal muscle of intense exercise mice （ P 〈 0. 01 ）. [Conclusion] Curcumin exhibited antioxidative protective role in intense exercise-induced injuries of skeletal muscle.

关 键 词：姜黄素 急性游泳训练 氧化应激 超微病理 

分 类 号：R285.5[医药卫生—中药学]