蜥蜴利什曼原虫表达柔嫩艾美耳球虫SAG2基因的保护效果检测  

Protective efficacy of recombinant Leishmania tarentolae expressing the SAG2 gene against Eimeria tenella challenge

作　　者：郭衍冰[1] 曹利利[1] 姚新华[1] 董航[1] 姜旭[1,2] 苑淑贤[1] GUO Yan-bing CAO Li-li YAO Xin-hua DONG Hang JIANG Xu YUAN Shu-xian(Jilin Institute of Animal Husbandry and Veterinary Sciences, Changchun, Jilin, 130062, China Agricultural College of Yanbian University, Yanji, Jilin, 133000, China)

机构地区：[1]吉林省畜牧兽医科学研究院,吉林长春130062 [2]延边大学农学院,吉林延边133000

出　　处：《吉林畜牧兽医》2017年第8期7-10,共4页Jilin Animal Husbandry and Veterinary Medicine

基　　金：吉林省科技发展计划项目20150204070NY;吉林省科技发展计划项目20160204020NY;吉林省科技引导计划项目20160417011CB

摘　　要：本试验将已构建的PLEXSY-neo2-SAG2在蜥蜴利什曼原虫中表达,以皮下注射的方式免疫雏鸡,经检测各组体液和细胞免疫水平、OPG、相对增重率及ACI等指标,对重组蛋白的抗球虫保护效果做出评价,为制备相关疫苗的研究奠定基础。In this study, expression the PLEXSY-neo2-SAG2 in Leishmania tarentolae which has been constructed successfully that immunized chickens via subcutaneous route, by detecting the chicken humoral and cellular immunity, OPG, relative growth rate, anticoccidial index and other indicators, provides evaluation of the anti-coccidiosis protective effect of recombinant protein and laid a foundation of further study on the development of relevant vaccine.

关 键 词：蜥蜴利什曼原虫 柔嫩艾美耳球虫 SAG2基因 保护效果 

分 类 号：S858.315.9[农业科学—临床兽医学]