山梨猕猴桃根化学成分及细胞毒活性研究  

Chemical constituents from roots of Actinidia rufa and their cytotoxicity

作　　者：黄初升[1,2] 马思远[1] 刘红星[1,2] 陆倩[1] 石灵高[3] 廖娜[3] 韦柳斌[3,2] HUANG Chu-sheng MA Si-yuan LIU Hong-xing LU Qian SHI Ling-gao LIAO Na WEI Liu-bin(College of Chemistry and Materials Science, Guangxi Teachers Education University, Nanning 530001, China Department of Pharmacy, Medicine College of Guangxi University of Science and Technology, Liuzhou 545006, Chin State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin 541000, China)

机构地区：[1]广西师范学院化学与材料科学学院,广西南宁530001 [2]广西师范大学省部共建药用资源化学与药物分子工程国家重点实验室,广西桂林541000 [3]广西科技大学医学院药学系,广西柳州545006

出　　处：《中国中药杂志》2017年第14期2714-2718,共5页China Journal of Chinese Materia Medica

基　　金：广西教育厅高校科研项目(KY2015YB178);广西师范大学省部共建药用资源化学与药物分子工程国家重点实验室项目(CMEMR2016-B16);广西科技大学博士科研项目启动基金项目(14Z10)

摘　　要：探究山梨猕猴桃Actinidia rufa根的化学成分。采用葡聚糖凝胶色谱法、薄层色谱硅胶色谱法、高效液相色谱法等方法分离得到9个化合物,鉴定为:2α,3β,19α,23,24-五羟基乌苏-12-烯-28-O-β-D-吡喃葡萄糖苷(1)、2α,3α,19α,24-四羟基乌苏-12-烯-28-O-β-D-吡喃葡萄糖苷(2)、2α,3α,24-三羟基乌苏-12-烯-28-酸(3)、2α,3α,24-三羟基齐墩果-12-烯-28-酸(4)、2α,3α,23,24-四羟基乌苏-12-烯-28-酸(5)、2α,3β,23,24-四羟基乌苏-12-烯-28-酸(6)、2α,3β,23-三羟基乌苏-12-烯-28-酸(7)、2α,3β,23-三羟基乌苏-12,20(30)-二烯-28-酸(8)、2α,3α,24-三羟基乌苏-12,20(30)-二烯-28-酸(9),其中化合物1和2为首次从该属植物中分离得到。细胞毒活性测试结果显示化合物2~4对人卵巢癌细胞株SKVO3和人甲状腺乳头状癌细胞株TPC-1具有强的细胞毒活性(IC50在10.99~16.41μmol·L^(-1)),而化合物3,4对人宫颈癌细胞株He La具有强的细胞毒活性,IC50分别为15.53,13.07μmol·L^(-1)。To investigate the chemical compounds from the roots of Actinidia rufa, nine compounds were isolated by various column chromatography on silica gel and Sephadex LH-20, and high performance liquid chromatography （HPLC）. Their structures were elucida- ted as 2α, 3β, 19α, 23, 24-pentahydroxyurs-12-en-28-oic acid-28-O-β-D-glucopyranoside （1）, 2α, 3α, 19α, 24-tetrahydroxyurs-12-en- 28-oic acid-28-O-β-D-glucopyranoside （2）, 2α, 3α, 24-trihydroxyurs-12-en-28-oic acid （3）, 2α, 3α, 24-trihydroxyolean-12-en-28-oic acid （4）, 2α, 3α, 23, 24-tetrahydroxyurs -12-en-28-oic acid （5）, 2α, 3β, 23, 24-tetrah-ydroxyurs-12-en-28-oic acid （6）, 2α, 3β, 23-trihydroxy-12-en-28-oic acid （7）, 2α, 3β, 23-trihydroxyurs-12, 20（30）-dien-28-oic acid （8）, and 2α, 3α, 23-trihydroxyurs-12, 20 （30）-dien-28-oic acid （9）. Compounds 1 and 2 were isolated from the Actinidia genus for the first time. Compounds 2, 3, and 4 showed cytotoxic activity against human SKVO3 and TPC-1 cancer cell lines with IC50 values ranging from 10. 99 to 16. 41μmol . L-1 , compounds 3 and 4 have cytotoxic activity against human HeLa cancer cell line with IC50 values of 15.53 and 13. 07 μmol. L-1 , respectively.

关 键 词：猕猴桃属 山梨猕猴桃 三萜 抗肿瘤 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]