牛乳中乳房链球菌PCR检测方法的建立  

Development of PCR for Streptococcus uberis Detection in Milk of Dairy Cows

作　　者：刁巧虹 卞国志[4,2] 王娟 罗蔼剑 张莹[2,3] 高潇祎 贾坤[2,3] 孙凌霜[2,3] 袁建丰 

机构地区：[1]广东海大畜牧兽医研究院有限公司,广东广州511400 [2]华南农业大学兽医学院,广东广州510642 [3]广东省兽医临床重大疾病综合防控重点实验室,广州广东510642 [4]广东海大集团股份有限公司,广东广州511400

出　　处：《动物医学进展》2017年第8期14-18,共5页Progress In Veterinary Medicine

基　　金：广州市科技计划项目(201510010250);广东省科技计划项目(2014A020208052;2015B020203005;2015A020224039);广东省兽医临床重大疾病综合防控重点实验室(2013A061401013);广东省现代农业科技创新联盟建设项目(2016LM2150);广州市珠江科技新星(201610010073)

摘　　要：奶牛乳房炎是危害奶牛业最常见的疾病之一,对奶牛业造成了严重的经济损失。引起奶牛乳房炎的病原菌较多,其中乳房链球菌是常见的致病菌之一。为快速准确地检测样品中的该菌,参照GenBank公布的乳房链球菌pauA基因序列设计引物,建立了乳房链球菌的PCR检测方法。对460份临床奶样进行检测,传统细菌分离方法共检出11份(2.39%)阳性奶样;PCR方法共检出40份(8.70%)阳性奶样,2种方法的吻合率为93.7%。结果显示,建立的乳房链球菌PCR检测法敏感性高,特异性强,适用于临床检测。Mastitis is one of the most popular diseases that causes significant economic loss in dairy cattle industry. Streptococcus uberisis is one of the pathogens leading to mastitis in dairy cows. According to the sequence of Streptococcus uberis pauA gene published by GenBank, one pair of primers were designed to establish a method for the detection of Streptococcus uberis. And 460 milk samples were detected by this method, a total of 11 （2.39%） of positive milk samples were detected by traditional bacterial isolates, while 40（8.7%） of positive milk samples were detected by PCR, the coincidence rate between the 2 methods was 93.7%. The results showed that the new method is sensitive to detect Streptococcus uberis in clinical milk samples and had the advantages of high sensitivity and good specificity.

关 键 词：奶牛乳房炎 乳房链球菌 聚合酶链反应 

分 类 号：S852.611[农业科学—基础兽医学] S858.23[农业科学—兽医学]