蛇代谢综合征继发感染细菌的分离鉴定及致病性试验  

Identification and Pathogenicity of Bacteria Isolated from Snake Metabolic Syndrome

作　　者：韩林梅[1] 苏接瑜 覃炳雄 曾芸[1] 李军[2,3] 廖河康 

机构地区：[1]广西大学动物科学技术学院,广西南宁530004 [2]广西兽医研究所,广西南宁530001 [3]广西兽医生物技术重点实验室,广西南宁530001 [4]广西陆生野生动物救护研究中心与疫源疫病监测中心,广西南宁530001

出　　处：《动物医学进展》2017年第8期43-48,共6页Progress In Veterinary Medicine

基　　金：南宁市2016年科技项目(20163355)

摘　　要：近年来,广西人工饲养的蛇发生代谢障碍综合征后,病死率较高,为查清原因,对临床接诊的18例蛇代谢障碍综合征病例的心、肝、脾等脏器病料进行细菌分离鉴定,并测定分离菌对小鼠和蛇的致病力。结果从18例病蛇的不同组织器官中分离到10株细菌,PCR结合生化试验方法,鉴定出4种细菌,分别为粪肠球菌、克氏葡萄球菌、缓慢葡萄球菌和弗格森氏大肠埃希菌,并对其进行种系发育关系分析。所鉴定的4种细菌对昆明小鼠和蛇的致病力差异较大,克氏葡萄球菌和弗格森氏大肠埃希菌对昆明小鼠有较强致病性,粪肠球菌和缓慢葡萄球菌对昆明小鼠致病力较弱;克氏葡萄球菌对眼镜蛇有致病性,其余菌对王锦蛇、眼镜蛇、滑鼠蛇均无致病性。The snake metabolic syndrome was broken in artificial feeding snakes in Guangxi recently. For its high mortality, the pathogens of snake metabolic syndrome were investigated. Bacteria were isolated and identified from heart, liver, spleen of 18 cases of snake metabolic syndrome, and their pathogenicity were tested in mice and snakes.The results showed that 10 strains of bacteria were isolated from different organs of sick snakes.Enterococcus faecalis , Staphylococcus kloosii , Staphylococcus lentus , Escherichia fer gusonii were identified by PCR and biochemical tests. The virulences of 4 species of bacteria to mice and snakes were different.Staphylococcus kloosii and Escherichia fergusonii had strong pathogenicity to mice,howev- er,Enterococcus faecalis and Staphylococcus lentus appeared low virulence.Enterococcus faecalis , Staphy- lococcus lentus and Escherichia fergusonii were avirulent to Elaphe carinata, cobra and Ptyas mucosus, except that Staphylococcus kloosii was virulent to cobra.

关 键 词：代谢综合征 细菌 鉴定 致病力 蛇 

分 类 号：S865.32[农业科学—野生动物驯养]