CD8^+T细胞表位鉴定技术研究进展  

Progress on CD8^+T Cell Epitope Mapping Techniques

作　　者：樊淑华[1,2] 王永立[1] 

机构地区：[1]周口师范学院生命科学与农学学院,河南周口466001 [2]中国农业大学动物医学院,北京100193

出　　处：《动物医学进展》2017年第8期85-89,共5页Progress In Veterinary Medicine

基　　金：国家863计划项目(2013AA102503);河南省科技厅科技发展计划项目(162102310587);周口师范学院高层次人才启动项目(700-70161)

摘　　要：CD8^+T细胞表位通过MHCⅠ分子呈递到细胞表面,激活CTL细胞从而介导并调节机体抗内源性抗原的免疫应答。MHC-peptide稳定性试验通过TAP缺陷的RMAS细胞转染克隆在细胞水平鉴定CD8^+T细胞表位与MHCⅠ分子的亲和力;酶联免疫斑点技术(ELISPOT)通过检测IFN-γ的分泌水平评估抗原免疫后机体的细胞免疫应答能力,具有较高的敏感性和高效性。四聚体技术通过流式细胞仪分析抗原特异性CD8^+T细胞的表型和功能特征。论文介绍了CD8^+T细胞表位的特征和上述3种表位鉴定方法的基本原理和研究进展,为CD8^+T细胞表位的鉴定和表位疫苗的研制提供参考。CD8＋T cell epitopes submitted by MHC I molecules can stimulate CTL cells,and play a crucial role in mediating and regulating immunoreactions against endogenous antigens.The binding of peptides to MHCI molecule could be detected using a stably transfected clone of TAP-deficient RMA-S; The IFN- gamma secretory ELISPOT （enzyme-linked immunospot） assay was found to be more sensitive and cost- effective compared to other methods and widely used on the detection of cell immune responses to immunization; Flow cytometry was used to examine the surface markers and biological function of antigen-specific CD8＋ T cell with MHC class I peptide tetramers.This article reviewed the latest progress on the three techniques of experimental identification of CD8＋T cell epitopes and its application for facilitating the development of epitope vaccines.

关 键 词：T细胞表位 CD8+T细胞 表位预测 表位鉴定 

分 类 号：S852.43[农业科学—基础兽医学]