骨形成蛋白4对单侧输尿管梗阻小鼠肾组织白细胞介素-1β表达及细胞凋亡的影响  

Bone Morphogenetic Protein 4 on the Expression of IL-1β and Apoptosis in Mouse Models of Unilateral Ureteral Obstruction

作　　者：付强[1] 于会娜[1] 王婷[1] 余孔贵[1] 唐临飞[1] 封其华[2] Fu Qiang Yu Huina Wang Ting Yu Konggui Tang Linfei Feng Qihua(Jingxhou Hospital Affiliated to Tongfi Medical College, Huazhong University of Science and Technology, Hubei Jingzhou 434020, China Children＇s Hospital of Soochow University, Jiangsu Suzhou 215025, China)

机构地区：[1]华中科技大学同济医学院附属荆州医院,湖北荆州434020 [2]苏州大学附属儿童医院,江苏苏州215025

出　　处：《儿科药学杂志》2017年第8期1-3,共3页Journal of Pediatric Pharmacy

摘　　要：目的:探讨骨形成蛋白4(BMP4)对单侧输尿管梗阻(UUO)小鼠肾组织白细胞介素-1β(IL-1β)表达及细胞凋亡的影响。方法:将32只雄性C57BL/6小鼠随机分为生理盐水-假手术组、生理盐水-UUO组、BMP4抗体-假手术组、BMP4抗体-UUO组各8只,各组均于手术日起每天分别给予小鼠抗人BMP4抗体200μL/g或等量生理盐水腹腔注射,术后7 d处死小鼠取材。采用免疫荧光法观察正常小鼠肾脏组织BMP4的表达与分布;实时荧光定量PCR检测小鼠肾脏组织IL-1βmRNA的表达;TUNEL法检测细胞凋亡情况。结果:正常小鼠肾组织表达BMP4,主要分布于肾小管。BMP4抗体-假手术组和生理盐水-假手术组的IL-1βmRNA表达、细胞凋亡率比较差异均无统计学意义(P均>0.05);BMP4抗体-UUO组IL-1βmRNA表达、细胞凋亡率低于生理盐水-UUO组(P均<0.05)。结论:BMP4可能参与了梗阻性肾病肾间质炎症及细胞凋亡过程,有可能作为肾脏疾病的治疗靶点。Objective： To observe the effects of bone morphogenetic protein 4（ BMP4） on the expression of IL-1β and apoptosis in mouse models of unilateral ureteral obstruction（ UUO）. Methods： Thirty-two C57BL/6 mice were randomly divided into four groups：saline-sham operated group（ n = 8）,saline-UUO group（ n = 8）,BMP4 antibody-sham operated group（ n = 8）,and BMP4 antibodyUUO group（ n = 8）. Each group was given intraperitoneal injection of either saline or BMP4 antibody（ 200 μL/g） every day after operation for 7 days. The mice were sacrificed at 7 day to evaluate the expression of BMP4 in renal tissue of normal mouse by immunofluorescence. Fluorescent quantitative real-time PCR was used to detect the expression of IL-1β mRNA in each group. Besides,the renal cell apoptosis was evaluated by TUNEL-assay. Results： The expressions of BMP4 were mainly distributed in the renal tubules of mice. Real-time PCR showed that the expression of IL-1β mRNA and apoptosis rate in BMP4 antibody-sham operated group and saline-sham operated group were with no significant differences（ P〉0. 05）. The expression of IL-1β mRNA and apoptosis rate in BMP4antibody-UUO group were less than saline-UUO group（ P〈0. 05）. Conclusion： BMP4 participates in the process of renal interstitial inflammation and cell apoptosis in obstructive nephropathy,it can be used as a therapeutic target for renal disease.

关 键 词：骨形成蛋白4 输尿管梗阻 白细胞介素-1Β 细胞凋亡 

分 类 号：R593[医药卫生—内科学]