梨泡状溃疡类病毒的RT-PCR检测及序列分析  

RT-PCR detection and sequence analysis of Pear blister canker viroid infecting pear

作　　者：朱慧[1,2] 王国平[1,2] 杨作坤 文立红[1] 陈婕 徐文兴[1] 洪霓[1,2] ZHU Hui WANG Guo-ping YANG Zuo-kun WEN Li-hong CHEN Jie XU Wen-xing HONG Ni(College of Plant Science and Technology, Key Lab of Crop Disease Monitoring and Safety Control in Hubei, Huazhong Agricultural University, Wuhan 430070, China National Key Lab of Agromicrobiology, Huazhong Agricultural Uni- versity ,Wuhan 430070, China)

机构地区：[1]华中农业大学植物科技学院,湖北省作物病害监测与安全控制重点实验室,武汉430070 [2]华中农业大学,农业微生物学国家重点实验室,武汉430070

出　　处：《植物病理学报》2017年第4期558-562,共5页Acta Phytopathologica Sinica

基　　金：国家农业行业专项计划项目(nyhyzx201203076-03);农业产业技术体系(CARS-29-10)

摘　　要：梨泡状溃疡类病毒（Pear blister canker viroid,PBCVd）为马铃薯纺锤块茎类病毒科（Pospiviroidae）苹果锈果类病毒属（Apscaviroid）成员）。该类病毒在自然条件下主要侵染野生和栽培的梨和苹果,梨和榅桲多数品种感染PBCVd后无明显症状,Pear blister canker viroid （PBCVd） is a member of the genus Apscaviroid in the family Pospiviroi- dae.The viroid naturally infects pear （Pyrus spp.） and apple （Malus spp.）. In this study, for the first time, PBCVd was detected in seven pear trees from three provinces of China by using RT-PCR. Except the isolate XH consisting of 314 nucleotides （ nt）, the complete RNA of other six PBCVd isolates consisted of 312 nt. These isolates shared 98.4%-100%identity with each other and 88.7%-100%identity with previously reported isolates at the nucleotide level. Phylogenetic analysis revealed that these isolates, together with a reference isolate P2098T, clustered into the same group. These results provide important information for further understanding the PBCVd occurrence in pear plants and enhancing the monitoring of PBCVd in China.

关 键 词：泡状溃疡 RT-PCR检测 类病毒 序列分析 分离物 野生梨 二级结构 分子特性 扩增产物克隆 病毒属 

分 类 号：S432.41[农业科学—植物病理学]