番荔枝内酯体外抑制人胃癌细胞活性研究  

Inhibition of annonaceous acetogenins on cell activities of human gastric cancer cells in vitro

作　　者：叶健斌 温俊杰[1] 林彦青 吴丹琳[1] 胡冰心[1] 罗美群[1] 宁立军[1] 宁云山[1] 李妍[1] 

机构地区：[1]南方医科大学检验与生物技术学院生物治疗研究所,广东广州510515

出　　处：《中草药》2017年第14期2895-2901,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金面上项目(81470831);国家自然科学基金-广东省联合基金重点项目(U1401223);国家高技术研究发展技术("863"计划)(2014AA020909);广东省科技计划创新载体建设项目(2013B090800036);广东省科技计划项目(2015A040404021;2016B090919019);广东省大学生创新项目(201512121122;201612121100;201612121243;201612121034;201612121246);深圳市科技计划项目基础研究项目(JCYJ20160429102107498);深圳市卫生计生系统科研项目(201606028)

摘　　要：目的探究番荔枝内酯对人胃癌细胞体外活性的影响。方法分别采用MTT实验、黏附实验和划痕实验考察番荔枝内酯对4种人胃癌细胞AGS、MKN45、BGC823和SGC7901生长活性、黏附能力和迁移能力的影响。结果番荔枝内酯抑制人胃癌细胞AGS、MKN45、BGC823和SGC7901的生长并呈剂量依赖性,其IC50值分别为5.32、6.25、5.46、4.43μg/mL;抑制人胃癌细胞黏附能力并呈剂量依赖性;抑制人胃癌细胞的迁移能力并呈剂量依赖性。结论番荔枝内酯体外可以有效抑制人胃癌细胞的活性,有望被开发成治疗胃癌的候选药物。Objective To study the effect of annonaceous acetogenins（ACGs） on human gastric cancer cells in vitro. Methods After ACGs were administered to gastric cancer cells in vitro, the cell viability, cell adhesion ability and cell migration ability were assessed by MTT assay, adhesion assay and wound-healing assay, respectively. Results ACGs inhibited the cell viability, adhesion ability and migration ability in a dose-dependent manner in gastric cancer cells. Conclusion ACGs could inhibit cell activities of human gastric cancer cells in viro, and will be developed as a promising anticancer candidate and used in gastric cancer.

关 键 词：番荔枝内酯 人胃癌细胞 生长 黏附 迁移 AGS细胞 MKN45细胞 BGC823细胞 SGC7901细胞 

分 类 号：R285.5[医药卫生—中药学]