中空纤维支载离子液体液相微萃取检测尿液中阿片类毒品  

Determination of Opioid Drugs in Urine by Liquid Phase Microextraction Based on Ionic Liquid Auxiliary Hollow Fiber

作　　者：王丹[1,2] 

机构地区：[1]中国人民公安大学,北京100035 [2]中国刑事警察学院,沈阳110854

出　　处：《基因组学与应用生物学》2017年第7期2781-2786,共6页Genomics and Applied Biology

基　　金：国家自然科学基金(No.21205080);辽宁省教育厅项目(No.L2014461)共同资助

摘　　要：本研究建立以离子液体作为萃取溶液,聚丙烯中空纤维膜作为支载体,三相微萃取尿液中的目标物质,以期建立阿片类毒品的高效液相色谱检测方法(IL-HF-LPME-HPLC)。实验以吗啡、曲马多、可待因、美沙酮四种阿片类毒品为研究对象,优化出最佳的HF-LPME的萃取模式和条件为:萃取剂为3-辛基-3-甲基咪唑六氟磷酸离子液体加15%三辛基氧化膦;接收相均为p H为1,加入Na Cl为2 mol/L,搅拌速度为1 000 r/min,萃取时间为60 min。通过本优化的方法检测4种阿片类物质,获得了较高的富集倍数(98~178倍)和较低的检测限(10~50 ng/m L),相对标准偏差(RSD)均为小于10%。研究表明,中空纤维膜起到了很好的隔离复杂基质中的大分子干扰作用,具有十分良好的富集效果。A method for the determination of morphine, tramadol, codeine, methadone 4 opioid drugs in urine was developed using Ionic liquidauxiliary hollow fiber membrane liquid phase micro extraction coupled with high per- formance liquid chromate graphy. The optimized micro extraction conditions were as follows： 1-octyl-3-methylim- idazolium hexafluorophosphate ＋15% trioctylphosphine oxide was used as extraction solvent, the pH value of ac- ceptor phase was 11, the NaCl concentration in sample solutions was 2 mol/L, the stirring rate was 1 000 r/min and the extraction time was 60 min. By the methods, high enrichment factor （98-178 times） and the lower detection limit （below 10 ng/mL） were obtained. Relative standard deviation （RSD） was less than 10%. Hollow fiber membrane has played a very good barrier effect, effectively eliminate the interference of the matrix ofmacromolecular complex.

关 键 词：中空纤维 液相微萃取 离子液体 阿片类毒品 尿液 

分 类 号：R446.12[医药卫生—诊断学]