长顺绿壳蛋鸡绿壳基因SLCO1B3多态性分析  

Polymorphic Analysis of Oocyan Gene SLCO1B3 of Changshun Blue-Eggshell

作　　者：喻昌燕 谢健[1] 王洋[1] 林家栋[2] 陈眷华[2] 张福平[2] 

机构地区：[1]贵州大学生命科学学院,贵阳550025 [2]贵州大学动物科学学院,贵阳550025

出　　处：《基因组学与应用生物学》2017年第7期2816-2820,共5页Genomics and Applied Biology

基　　金：贵州省科技计划项目(黔科合NY[2015]3006-1号)基金资助

摘　　要：为了筛选出产绿壳蛋的纯合子基因型鸡群。本试验采用双重PCR方法检测长顺绿壳蛋鸡群体中EAV-HP在SLCO1B3基因中的插入频率,结果显示,该群体中包含3种基因型:N/N、LC/N和LC/LC,其基因型频率分别为5.23%、52.33%和42.44%;LC基因频率为68.60%,绿壳蛋频率达到94.77%。该插入位点在长顺绿壳蛋鸡群体中处于Hardy-Weinberg不平衡状态(p<0.01)。表明长顺绿壳蛋鸡群体拥有大量EAV-HP插入整合的个体,其基因纯合度较高,是优良的地方品种资源。This study aimed to screen a homozygous genotype group. Duplex PCR method was used to detect the insertion frequency of EAV-HP in SLCOIB3 gene of Changshun blue-eggshell chicken populations. The results showed that the group had three genotypes, N/N, LC/LC and LC/N, which genotype frequencies were 5.23%, 52.33% and 42.44%. The gene frequency of LC was 68.60%, and blue-eggshell frequency reached at 94.77%. The insertion site in Changshun blue-eggshell chicken was in Hardy-Weinberg disequilibrium （p 〈0.01）, which indicat- ed Changshun blue-eggshell chicken hadan amount of EAV-HP insertion which hadhigher homozygosity gene was an excellent breed resource.

关 键 词：长顺绿壳蛋鸡 EVA-HP SLCO1B3 

分 类 号：S831[农业科学—畜牧学]