沙冬青脱水素基因提高转基因紫花苜蓿的耐旱性  

AmDHN Gene from Ammopiptanthus mongolicus Improved the Drought Tolerance of Transgenic Alfalfa(Medicago sativa)

作　　者：聂利珍[1] 刘红葵[1] 李晓东[1] 孙杰[1] 房永雨[1] 

机构地区：[1]内蒙古农牧业科学院,呼和浩特010031

出　　处：《基因组学与应用生物学》2017年第7期2947-2953,共7页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31660686);内蒙古农牧业科学院科技创新基金项目(2016CXJJM07)共同资助

摘　　要：为鉴定转基因紫花苜蓿的耐旱能力,本研究以转基因紫花苜蓿T0代植株为材料,通过干旱胁迫,从转基因植株的表型、生理指标和分子水平等层面检测转基因植株的抗旱特性,试验表明,干旱胁迫后转基因苜蓿和对照的茎、叶片全部干枯,而复水后转基因苜蓿大部分恢复生长,对照几乎全部死亡。干旱胁迫后苜蓿叶片的Pro和MDA含量均随处理时间延长而逐渐增加,转基因植株叶片的Pro含量高于对照,而其MDA含量低于对照植株,且二者均达到了显著差异;转基因苜蓿的离体叶片失水率也明显低于对照。在20%PEG胁迫下,植株体内ProDH和P5CS基因的相对表达量都是先升后降,转基因植株和对照中ProDH和P5CS基因的表达量显著增加,且二者在转基因植株与对照间存在显著差异。这表明,在干旱胁迫条件下,由于AmDHN基因在紫花苜蓿中的过量表达,可能引起植株体内脯氨酸的大量积累,并进一步诱导脯氨酸合成相关基因的表达,最终引起植株抗旱性的提高,因此,推测转基因苜蓿的耐旱性比对照强。In order to identify the drought tolerance of transgenic alfalfa, we used alfalfa transgenic To plants as the experimental materials. Drought resistance of the transgenic plants was detected by their phenotype, physiological characteristic and molecular level under drought stress. The main results indicated that stems and leaves of transgenic plants and those in the control group seriously withered under drought stress. However, most transgenic plants revived normal growth, while the control group were almost all died after the rewatering. The content of proline and MDA in the leaves of alfalfa both increased gradually with the extending of treatment time under drought stress. The content of proline in transgenic plants was significantly higher than that in the control, while the content of MDA was significantly lower than the control group, and the both differences reached a significant level after drought treatment. In addition, the rate of water loss of isolated leaves in transgenic plants was significantly lower than that in the control under drought stress. Relative expressions of ProDH and P5CS gene both increased in early period, and then decreased under the stress of 20% PEG. Relative expression of P5CS and ProDtt in transgenic plants both enhanced significantly compared with the control plants, and the difference reached the significant level. These results implied that under drought stress, the over-expression ofA rnDHN gene in alfalfa might cause the accumulation of proline in plant, and further induce the expression of genes related toproline synthesis, resulting in the improvement of drought resistance in plants. We could speculate that the drought tolerance of the transgenic alfalfa enhanced significantly.

关 键 词：转基因紫花苜蓿 沙冬青脱水素基因 耐旱性 

分 类 号：S541.9[农业科学—作物学]