乌骨藤提取物对四氯化碳致大鼠慢性肝损伤的保护作用  

Protective effects of Marsdeniatenacissima extract on CCl_4-induced chronic liver injury in rats

作　　者：苏远[1] 张小磊[1] 章礼久[1] 

机构地区：[1]安徽医科大学第二附属医院消化内科,合肥230601

出　　处：《中国新药杂志》2017年第14期1643-1648,共6页Chinese Journal of New Drugs

基　　金：安徽省公益性研究联动计划资助项目(1501ld04046);安徽医科大学校科研基金资助项目(2015xkj127);安徽省自然科学基金资助项目(1708085QH192)

摘　　要：目的:探讨乌骨藤提取物对四氯化碳(CCl_4)致慢性肝损伤模型大鼠的保护作用。方法:50只SD大鼠随机分为空白对照组,模型组,乌骨藤低、中、高剂量(10,20,40 mg·kg^(-1))组。以CCl_4皮下注射每周2次制备慢性肝损伤模型。同时各给药组每天灌胃(ig)给药1次,连续6周。计算大鼠的肝脏和脾脏指数;检测血清ALT,AST和肝组织中SOD,MDA,GSH-Px和Hyp水平;HE染色法检测肝组织病理变化;ELISA法检测大鼠血清IL-2水平;流式细胞术检测大鼠外周血T淋巴细胞亚群(CD3^+,CD4^+,CD8^+)的变化情况。结果:给予不同剂量乌骨藤治疗慢性肝损伤模型大鼠后,大鼠肝脾指数、血清ALT,AST,MDA和Hyp水平均不同程度降低,SOD,GSH-Px,IL-2水平升高,升高或降低的差异均以乌骨藤高剂量组尤为显著(P<0.01,P<0.05);乌骨藤显著改善大鼠肝组织病理损伤;大鼠外周血CD3^+和CD4^+亚群细胞比例下降,CD8^+细胞比例上升,CD4^+/CD8^+比值降低。结论:乌骨藤提取物对CCl4诱导的大鼠慢性肝损伤有保护作用,作用可能与其平衡T淋巴细胞亚群,调节免疫功能有关。Objective： To investigate the protective effects of Marsdeniatenacissima extract on CC14-induced chronic liver injury in rats. Methods： Fifty SD rats were randomly divided into the following groups： normal control group, model group, low-, middle- and high-dose M. tenacissima groups （ 10, 20, 40 mg· kg- 1 ） CC14 was given to rats by subcutaneous injection to make liver injury model. At the same time, the treatment groups were intragastricly administered with M. tenacissima twice a week for six weeks. The liver and spleen indexes of rats were calculated. The activities of ALT, AST in serum and SOD, MDA, GSH-Px and Hyp in liver tissues were tested. Histopathology of liver was observed by HE staining. The level of IL-2 in serum was tested by ELISA. The T lymphocytes （ CD3 ＋ , CD4＋, CD8 ＋） in blood were detected by using flow cytometer. Results ： Compared with the model group, M. tenaclssima-treated groups showed decreases in the activities of ALT, AST, SOD, MDA, GSHPx and Hyp, and an increase in IL-2 level. Moreover, the pathological damage of liver tissue was obviously improved by M. tenacissima. Furthermore, the proportions of CD3 ＋, CD4＋, CD4＋/CD8＋ were reduced, while that of CD8 ＋ cells was up-regulated. Conclusion： Marsdeniatenacissima extract has protective effect on CC14-induced chronic liver injury in rats, which might be associated with its regulation of immunological function by counterbalance the T lymphocyte subsets.

关 键 词：乌骨藤提取物 四氯化碳 慢性肝损伤 T淋巴细胞亚群 白介素2 

分 类 号：R285[医药卫生—中药学] R932[医药卫生—中医学]