辽宁沿海日本海马线粒体控制区序列变异及其在海龙科鱼类系统分析中的应用  

Sequence variation of mtDNA control region in Hippocampus japonicus inhabiting Liaoning coast and its applicability as a marker for phylogenetic analysis

作　　者：李玉龙[1] 王彬[1] 王爱勇[1] 于旭光[1] 李轶平[1] 韩振华[1] 付杰[1] 董婧[1] LI Yulong WANG Bin WANG Aiyong YU Xuguang LI Yiping HAN Zhenhua FU Jie DONG Jing(Liaoning Ocean and Fishery Science Research Institute, Liaoning Key Laboratory of Marine Biological Resources and Ecology, Dalian 116023, China)

机构地区：[1]辽宁省海洋水产科学研究院,辽宁省海洋生物资源与生态学重点实验室,辽宁大连116023

出　　处：《水产学报》2017年第7期1073-1082,共10页Journal of Fisheries of China

基　　金：海洋公益性行业科研专项(201405010);辽宁省海洋与渔业科研项目(201401)~~

摘　　要：为研究日本海马野生群体的种质资源及遗传多样性状况,实验采用PCR扩增法获得日本海马线粒体DNA的控制区序列片段,同时利用GenBank数据库中已有的14种海龙科鱼类控制区同源序列对其进行序列比较及系统进化分析。结果显示,日本海马控制区序列片段长度为557~558 bp,其A、T、G、C 4种碱基的平均含量分别为34.3%,29.7%,14.1%,21.9%。在控制区序列片段中,共检测到16个多态性核苷酸位点,定义了16种单倍型,其核苷酸多态度和单倍型多态度都较低(π=0.0032±0.0021,h=0.70±0.02)。利用GenBank数据库中已有的海马控制区同源序列,采用邻接法、最大似然法和贝叶斯法构建了分子系统树。结果显示,采用最大似然法和贝叶斯法构建的分子系统树拓扑结构与邻接法构建的分子系统树拓扑结构不完全相同但基本一致,系统进化分析结果与形态分类学的观点一致。研究表明,线粒体DNA控制区序列在海龙科不同阶元间变异较大,适合于海龙科鱼类种间、群体水平的研究以及作为系统进化分析的分子标记。To study the germplasm resources and genetic diversity of the wild population of Hippocampus japonicus, the mitochondrial DNA control region fragments were obtained from wild populations by PCR amplification. The homologous sequences of other Syngnathidae fishes from GenBank were also included in this study. PCR amplification products of 557–558 bp CR fragments were obtained, and the average contents of A, T,G and C were 34.3%, 29.7%, 14.1%, 21.9%, respectively. A total of 16 polymorphic nucleotide sites were detected, which defined 16 haplotypes. The variation level was low as H was 0.70±0.02 and π was 0.0032±0.0021.With the aid of the homologous sequences retrieved from GenBank, phylogenetic trees were constructed based on neibour-joining（NJ）, maximum-likelihood（ML） and Bayesian inference（BI） methods to build phylogenetic relationships within the Syngnathidae fishes. The phylogenic analysis showed that the topology of phylogenetic trees were almost consistent with each other, although the NJ tree and the ML/BI tree were somewhat different.The phylogenic analyses of CR gene sequences were also consistent with morphological taxonomy. Based on the results, several conclusions were drawn as follows：（1） CR is an appropriate marker for the seahorse species identification and population genetic analysis;（2） CR is highly divergent among different Syngnathidae fishes and could be an appropriate marker for molecular systematic studies. This study is expected to provide important information for the protection and utilization of H. japonicus resources in China.

关 键 词：日本海马 海龙科 线粒体DNA 控制区序列 序列变异 系统分析 

分 类 号：Q785[生物学—分子生物学] S965[农业科学—水产养殖]