红景天苷对Aβ_(1-40)诱导PC12细胞损伤的保护作用及机制  

Protective Effect of Salidroside on PC12 Cell Injury Induced by Aβ_(1-40) and the Mechanism

作　　者：黄旭娇 邢三丽[1] 陈川[1] 郁志华[1] 陈久林[1] Huang Xujiao Xing Sanli Chen Chuan Yu Zhihua Chen Jiulin(Shanghai Geriatric Institute of Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200031, P. R. China)

机构地区：[1]上海市中医老年医学研究所,上海中医药大学,上海200031

出　　处：《老年医学与保健》2017年第3期189-192,共4页Geriatrics & Health Care

基　　金：国家自然科学基金(81503626);上海市卫生和计划生育委员会青年基金(201540254)

摘　　要：目的观察红景天苷对Aβ_(1-40)诱导PC12细胞损伤的保护作用,探讨改善能量代谢障碍对阿尔茨海默病发生发展的干预机制。方法利用Aβ_(1-40)诱导PC12细胞损伤模型,采用CCK-8法检测细胞活力,ATP试剂盒(比色法)检测ATP含量,免疫荧光法和Western blot法检测相关蛋白PGC-1α的表达。结果红景天苷能够抑制Aβ_(1-40)诱导的PC12细胞活力下降(P<0.05),增加ATP含量(P<0.01),免疫荧光和Western blot结果均显示红景天苷能够上调PC12细胞的PGC-1α蛋白表达(P<0.05)。结论红景天苷可能通过上调能量代谢相关蛋白PGC-1α的表达,从而提高ATP含量来抑制Aβ_(1-40)诱导PC12细胞的损伤,对阿尔茨海默病的治疗具有一定的应用价值。Objective To observe the effect of salidroside on PC 12 cell injury induced by Aβ1-40 and to explore its mechanism of improving energy metabolism disorder in the development of Alzheimer＇s disease.Methods PC12 cells were treated with Aβ1-40to build an AD cell model;CCK-8 method was applied in the measurement of cell viability and an ATP assay kit in the measurement of adenosine triphosphate（ATP） level;the expression level of PGC-1α was detected via immunofluorescence assay and Western blot analysis.Results Salidroside inhabited the decrease of PC12 cell viability induced by Aβ1-40（P〈0.05） and increased the level of ATP（P〈0.01）;the results of immunofluorescence assay and Western blot analysis indicated that salidroside up-regulated the expression of PGC-1α（P〈0.05）.Conclusions Salidroside may inhibit Aβ1-40-induced PC12 cells injury by up-regulating the expression of PGC-1α,an energy metabolism related protein,and increasing the level of ATP,it may be of certain curative effect on Alzheimer＇s disease

关 键 词：β-淀粉样蛋白1-40 阿尔茨海默病 红景天苷 三磷酸腺苷 

分 类 号：R285[医药卫生—中药学]