五味子乙素调控NF-κB通路对顺铂致HK-2细胞炎症损伤的保护作用  

Protective Effect of Schisandrin B for Protecting Cisplatin-Induced Inflammatory Damage of HK-2 Cells Through Regulating NF-κB Pathway

作　　者：李佳[1] 尹一子[1] 简晓顺[1] 林蔚[1] Li Jia Yin Yizi Jian Xiaoshun Lin Wei(Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong, China 510095)

机构地区：[1]广州医科大学附属肿瘤医院,广东广州510095

出　　处：《中国药业》2017年第12期11-14,共4页China Pharmaceuticals

基　　金：广州医科大学博士启动项目[2013C64]

摘　　要：目的研究五味子乙素对顺铂致HK-2细胞炎症损伤的保护作用及相关机制。方法以不同浓度的五味子乙素及顺铂干预人肾小管上皮HK-2细胞,CCK-8法检测细胞活性,Hoechst 33258荧光染色法观察细胞形态变化,Western blot法检测NF-κB活化,酶联免疫吸附试验(ELISA)法检测细胞上清液中炎性细胞因子[肿瘤坏死因子-α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)]水平,实时荧光聚合酶链反应(RT-PCR)法检测细胞中TNF-α,IL-1β,IL-6的m RNA表达。结果与对照组相比,顺铂组细胞活性显著下降,细胞形态明显改变;五味子乙素呈浓度依赖性地减轻顺铂的细胞毒性作用。同时,五味子乙素能显著抑制顺铂所致NF-κB活化及炎性细胞因子(TNF-α,IL-1β,IL-6)在细胞上清液中及m RNA表达的升高。结论五味子乙素能通过调控NF-κB通路对抗顺铂所致肾小管上皮细胞的炎症损伤。Objective To investigate the protective effect of schisandrin B against Cisplatin-induced inflammation damage in HK-2 cells and its related mechanism. Methods After treated with various concentrations of schisandrin B and Cisplatin,viability of HK-2 cells was determined by CCK-8 assay;cellular morphology was evaluated by Hoechst 33258 staining;activation of NF-κB was detect-ed by Western blot;levels of TNF-α,IL-1β,IL-6 in supernatants were measured by ELISA,mRNA expressions of TNF-α,IL-1β, IL-6 were detected by RT-PCR. Results Compared with the control group,the cell activity of the cisplatin group decreased signifi-cantly,and the cell morphology changed significantly. Schisandrin B dose-dependently reduced the cytotoxicity induced by cisplatin,at the same time,schisandrin B significantly inhibited the activation of NF-κB induced by Cisplatin and reversed the increase of levels of TNF-α,IL-1β,IL-6 in supernatants and mRNA expressions. Conclusion Schisandrin B can against Cisplatin-induced inflammation damage in HK-2 cells through regulating NF-κB pathway.

关 键 词：五味子乙素 顺铂 肾毒性 炎性反应 NF-ΚB通路 HK-2细胞 

分 类 号：R285.5[医药卫生—中药学] R287.1[医药卫生—中医学]