麻杏止咳糖浆含量测定方法研究  

Content Determination of Maxingzhike Syrup

作　　者：李冬梅 庄爱爱 苏建 赵振霞 刘永利 Li Dongmei Zhuang Aiai Su Jian Zhao Zhenxia Liu Yongli(Hebei Institute for Drug Control, Shijiazhuang, Heibei, China 050011 School of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Heibei, China 050016)

机构地区：[1]河北省药品检验研究院,河北石家庄050011 [2]河北科技大学化学与制药工程学院,河北石家庄050016

出　　处：《中国药业》2017年第12期27-29,共3页China Pharmaceuticals

基　　金：国家药品标准提高研究项目[项目编号:95]

摘　　要：目的建立测定麻杏止咳糖浆中盐酸麻黄碱、盐酸伪麻黄碱含量的高效液相色谱法。方法色谱柱为Boston Green ODS C18柱(250 mm×4.6 mm,5μm),以甲醇-0.1%磷酸(4∶96)为流动相,柱温为30℃,流速为1 mL/min,检测波长为210 nm。结果盐酸麻黄碱与盐酸伪麻黄碱进样量分别在0.086 4~8.64μg和0.082 72~8.272μg范围内与峰面积线性关系良好(r=0.999 9);平均加样回收率分别为96.75%和97.40%,RSD分别为1.38%和1.06%(n=9)。结论该方法操作简便、专属性强、准确度高、重复性好,可用于麻杏止咳糖浆中盐酸麻黄碱与盐酸伪麻黄碱的含量测定。Objective To establish an HPLC method for content determination of ephedrine hydrochloride and pseudoephedrine hydrochlo-ride in Maxingzhike Syrup. Methods The Boston Green ODS C18 column（250 mm × 4. 6 mm,5 μm） was adopted with methonal-0. 1%H3PO4（4 ：96）as the mobile phase,the column temperature was 30 ℃,the flow rate was 1. 0 mL/min and the detective wave-length was 210 nm. Results The calibration curve was linear at the ranges of 0. 0864-8. 64 μg,0. 08272-8. 272 μg for ephedrine hydrochloride and pseudoephedrine hydrochloride（r=0. 9999）,the average recoveries were 96. 75% and 97. 40%,the RSDs were 1. 38% and 1. 06%（n=9）. Conclusion The method is simple,accurate,and reproducible,which can be used as the content determina-tion of the ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxingzhike Syrup.

关 键 词：高效液相色谱法 麻杏止咳糖浆 盐酸麻黄碱 盐酸伪麻黄碱 含量测定 

分 类 号：R286.0[医药卫生—中药学] R284.1[医药卫生—中医学]