miR-126-5p在沙子岭猪睾丸组织差异表达分析及功能预测  

Differential Expression and Function Prediction of miR-126-5p in Shaziling Boar Testis

作　　者：董莲花 冉茂良[1,2] 翁波[1,2] 李智[1,2] 吴买生 陈斌[1,2] DONG Lian-hua RAN Mao-liang WENG Bo LI Zhi WU Mai-sheng CHEN Bin(College of Animal Science ＆ Technology, Hunan Agricultural University, Changsha 410128, China Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, Changsha 410128, China X iangtan Bureau of Animal Husbandry and Veterinary Medicine and Aquatic Product, Xiangtan 411104, China.)

机构地区：[1]湖南农业大学动物科学技术学院,湖南长沙410128 [2]畜禽遗传改良湖南省重点实验室,湖南长沙410128 [3]湘潭市畜牧水产局,湖南湘潭411104

出　　处：《家畜生态学报》2017年第7期20-25,共6页Journal of Domestic Animal Ecology

基　　金：国家现代农业产业技术体系建设专项资金(CARS-36)

摘　　要：利用实时荧光定量PCR(Real-time PCR)检测miR-126-5p在沙子岭猪睾丸组织8个不同发育时期(E90、D1、D30、D60、D90、D120、D150、D180)表达量,以了解其在动物睾丸发育及精子生成中的作用。利用miRanda、TargetScan和mirWalk 3个数据库预测miR-126-5p靶基因并取交集,利用David在线软件分析miR-126-5p靶基因的生物功能。结果表明,miR-126-5p在胚胎期(E90-D1)睾丸组织中高表达,而在生长期(D1-D90)表达量下调,但在沙子岭猪性成熟(D120)后表达量又上调,其中D1表达量最高,D90表达量最低,且二者与其他7个时期均差异极显著(P<0.01);D30、E90、D180相互之间差异不显著(P>0.05),且分别与其余各时期差异极显著(P<0.01);D120与D30差异显著(P<0.05),与其余各时期差异极显著(P<0.01);D150与D60之间差异不显著(P>0.05),二者分别与其余各时期差异极显著(P<0.01)。miR-126-5p靶基因参与细胞增殖、凋亡、分化、细胞通讯、迁移及蛋白质转运等生物学过程(P<0.001);靶基因分子功能包括蛋白二聚体活性、配体依赖性核受体活性(P<0.001);KEGG分析显示miR-126-5p靶基因参与TGF-β、MAPK、细胞黏附等信号通路(P<0.01)。结合miR-126-5p在睾丸组织不同发育时期表达差异结果及其靶基因功能预测结果,miR-126-5p可能参与沙子岭猪生殖细胞分化及精子生成调控,本研究为miR-126-5p在动物精子生中的作用及其调控机制的研究提供依据。In this study, real-time fluorescence quantitative PCR was performed to detect miR-126-Sp expression levels in eight different developmental stages （E90, D1, D30, D60, Dg0, D120, D150, D180） of Shaziling pig testis to explore the role of miR-126-5p in animal testicular development and spermatogene- sis. The miRanda, TargetScan and mirWalk databases were used to predict the target genes of miR-126- 5p, then the intersection from the three databases were taken to analyze the biological function of target genes by making use of David online software. The Real-time PCR results showed that miR-126-5p highly expressed in embryonic period （E90-D1） and with down-regulated expression in growth periods（D1-D90）, while up-regulated in the sexual maturity （D120）. The D1 and D90 was the highest and the lowest expres- sion level, and they were extremely significant（P〈0.01） with the other seven periods; the differences a- mong D30, E90, D180 were not significant （P〈0.05）, and they had extremely significant differences （P d0.01） with the rest of the periods; the difference between D120 and D30 was significant （P〈0.05）, and the difference between D120 and the other periods was extremely remarkable （P〈0.01）; there had no difference（P〉0.05） between D150 and D60, but they had bally distinct difference with the rest of periods respectively （P〈0.01）. The target genes of miR-126-5p involved in cell proliferation, apoptosis, differen- tiation, cell communication, migration and protein transport biological processes （P〈0. 001）, the molecu- lar functions including protein dimerization activity and ligand-dependent nuclear receptor activity （P 0. 001）, KEGG pathway analysis results showed that miR-126-5p involved in TGF-t3 and MAPK signaling pathway, focal adhesion （P〈0.01）. Combined the results of miR-126-5p expression differences at differ- ent developmental stages of testicular tissue with target genes function prediction, miR-126-5p may be in- volved in the regulati

关 键 词：沙子岭猪 miR-126-5p 生物信息学分析 精子生成 

分 类 号：S811.5[农业科学—畜牧学]