碧玉兰无菌播种快繁技术研究  

Study on Rapid Propagation Technology of aseptic seeding of Cymbidium lowianum(Rchb.f.) Rchb.f.

作　　者：李洪超[1,2] 汪旭[1] 张玉洁[3] LI Hongchao WANG Xu ZHANG Yujie(School of Life Science,Yunnan Normal University, Kunming 650500,Yunnan, China Babu Middle School, Malipo County 663605, Yunnan, China School of Resources and Environmem, Wenshan University, Wenshan 663099, Yunnan, China)

机构地区：[1]云南师范大学生命科学院,云南昆明650500 [2]麻栗坡县八布中学,云南麻栗坡663605 [3]文山学院环境与资源学院,云南文山663099

出　　处：《文山学院学报》2017年第3期12-14,共3页Journal of Wenshan University

摘　　要：以碧玉兰的种子为外植体进行组织培养。结果表明:碧玉兰种子萌发的最佳配方为:MS+NAA 0.5 mg/L+AC 2.0 g/L+蔗糖30 g/L+椰汁100 g/L,播种后50 d左右,可以形成绿色的原球茎(PLB);原球茎分化成幼苗的最佳配方为:1/2MS+6-BA 0.5 mg/L+KT 0.5 mg/L+蔗糖30 g/L+马铃薯100 g/L;生根并形成幼苗最佳的配方为:1/2MS+6-BA 0.2 mg/L+NAA 0.5 mg/L+AC 2.0 g/L+香蕉泥50 g/L。Rapid proliferation was done with seeds of Cymbidium lowianum（Rchb.F.） Rchb.F. as explant tissue. Results showed that the seeds can form protocorm in MS medium ＋ NAA0. 5 mg/L ＋ AC1.0 g/L ＋ sugar 30 g/L ＋ coconut milk 100 g/L 50 days after sowing. The best culture medium for differentiation of seedlings from protocorm is 1/2 ms ＋ 6-BA0.5 mg/L ＋ KT0.5 mg/L ＋ sugar 30 g/L ＋ 100 g/L potato. Differentiated seedlings can be induced to form complete plant in the medium containing 1/2 MS ＋ 6 – BA 0.2 mg/L ＋ NAA 0.5 mg/L ＋ AC2.0g/L＋ Banana juice 50g/L.

关 键 词：碧玉兰 外植体 组织培养 

分 类 号：S685.153.8[农业科学—观赏园艺]