DNA甲基化检测方法及其法医学应用研究进展  

Research Progress on the Detection Method of DNA Methylation and Its Applica-tion in Forensic Science

作　　者：聂燕钗[1] 俞丽娟[1] 管桦[1] 赵颖[1] 荣海博[1] 姜伯玮[1] 张涛[1] 

机构地区：[1]公安部第一研究所,北京100022

出　　处：《法医学杂志》2017年第3期293-300,共8页Journal of Forensic Medicine

摘　　要：DNA甲基化是表观遗传标记的重要组成部分,参与基因表达的调控,在生物发育、衰老以及肿瘤学等领域受到广泛关注。由于具有相对稳定性、可遗传、含量丰富、随龄变化等特点,在法医学领域,DNA甲基化可以作为DNA序列相关经典遗传标记的有效补充,用于年龄推断、组织体液来源检测、同卵双生子的鉴定等。DNA甲基化的检测方法主要有基于甲基化敏感限制性内切酶、重亚硫酸盐转化以及甲基化CpG结合蛋白等原理而建立的一系列方法,近年研究表明,第三代测序技术也可用于DNA甲基化检测。本文就DNA甲基化检测方法及在法医学领域的应用研究进行回顾与综述,以期为法医学实践提供参考。As an important part of epigenetic marker, DNA methylation involves in the gene regulation and attracts a wide spread attention in biological auxology, geratology and oncology fields. In forensic science, because of the relative stable, heritable, abundant, and age-related characteristics, DNA methylation is considered to be a useful complement to the classic genetic markers for age-prediction, tissueidentification, and monozygotic twins＇ discrimination. Various methods for DNA methylation detection have been validated based on methylation sensitive restriction endonuclease, bisulfite modification and methylation-Cp G binding protein. In recent years, it is reported that the third generation sequencing method can be used to detect DNA methylation. This paper aims to make a review on the detection method of DNA methylation and its applications in forensic science.

关 键 词：法医遗传学 DNA甲基化 综述 双生 单卵 三代测序 

分 类 号：DF795.2[医药卫生—法医学]