诱导HO-1表达对慢性间歇性缺氧下INS-1细胞的影响  

Effects of induced HO-1 expression on INS-1 cells in chronic intermittent hypoxia

作　　者：徐健[1] 李小莉[1] 李一禄[1] 陈济明[1] 

机构地区：[1]广东省深圳市南山区人民医院呼吸内科,518000

出　　处：《中国实用医刊》2017年第13期32-34,共3页Chinese Journal of Practical Medicine

摘　　要：目的 探讨诱导HO-1表达对慢性间歇性缺氧下INS-1细胞的影响.方法 通过不同浓度钴原卟啉（CoPP）作用于慢性间歇性缺氧下INS-1细胞,观察胰岛素、超氧化物歧化酶（SOD）、丙二醛（MDA）水平,采用RT-PCR检测HO-1 mRNA表达.结果 随着CoPP浓度增高,胰岛素、SOD、HO-1表达逐渐增高,而MDA逐渐降低,当CoPP浓度达到40 μmol/L时作用最强（P〈 0.05）.结论 慢性间歇性缺氧下诱导INS-1细胞HO-1表达,对细胞起一定保护作用.Objective To investigate the effect of induced HO-1 expression on INS-1 cells in chronic intermittent hypoxia.Methods INS-1 cells were observed under different concentrations of CoPP in chronic intermittent hypoxia, and the levels of insulin, SOD and MDA were observed.Using RT-PCR to detect the expression of mRNA HO-1.Results With the increasing of CoPP concentration, the expression of insulin, HO-1 and SOD gradually increased, while MDA decreased.When the concentration of CoPP reached 40 μmol/L, the strongest effect were observed.Conclusions Induced the expression of HO-1 in INS-1 cells in chronic intermittent hypoxia has a protective effect on the cells.

关 键 词：慢性间歇性缺氧 INS-1细胞 钴原卟啉 HO-1 HO-1 

分 类 号：R766[医药卫生—耳鼻咽喉科]