极低频磁场对小鼠胚胎成纤维细胞中隐花色素生物节律的影响  

Influence of extremely low-frequency magnetic field on circadian rhythm of cryptochrome in mouse embryonic fibroblasts

作　　者：孙兆钰 耿读艳[1] 陈传芳[2] 汪平平[2] 宋涛[2] 

机构地区：[1]河北工业大学、河北省电磁场与电器可靠性省部共建重点实验室,天津300130 [2]中国科学院电工研究所、北京市生物电磁学重点实验室

出　　处：《中华劳动卫生职业病杂志》2017年第6期459-462,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：基金项目：国家自然科学基金（51477169）

摘　　要：目的 研究极低频磁场对磁感应基因隐花色素（Cry）在小鼠胚胎成纤维细胞中的周期性表达情况.方法 实验分为磁场组和假暴露组,磁场组利用马血清刺激小鼠胚胎成纤维细胞,然后暴露于50 Hz、0.3 mT的极低频磁场中48 h;假暴露组同样利用马血清刺激小鼠胚胎成纤维细胞,置于假暴露线圈中48 h.收集小鼠胚胎成纤维细胞,提取总RNA并反转录成cDNA,利用实时荧光定量反转录聚合酶链式反应（RT-PCR）检测磁场组和假暴露组细胞中隐花色素和Period基因的转录周期变化.结果 与假暴露组比较,磁场组小鼠胚胎成纤维细胞暴露于磁场0、12、24和48 h后,细胞增殖率变化差异均无统计学意义（P〉0.05）.假暴露组和磁场组隐花色素基因表达均呈节律性变化,且与假暴露组比较,磁场组小鼠胚胎成纤维细胞隐花色素基因节律周期明显缩短,差异有统计学意义（t=2.57,P〈0.05）.假暴露组和磁场组小鼠胚胎成纤维细胞Period基因均呈节律性周期表达,但差异无统计学意义（t=0.70,P〉0.05）.结论 极低频磁场能够明显缩短小鼠胚胎成纤维细胞中磁感应基因隐花色素的生物节律,而Period基因的生物节律改变不明显.Objective To investigate the influence of extremely low-frequency magnetic field on periodical expression of cryptochrome （Cry） gene in mouse embryonic fibroblast NIH3T3 cells. Methods The NIH3T3 cells were divided into magnetic field group and sham-exposure group. The NIH3T3 cells in the magnetic field group were stimulated by horse serum and then exposed to an extremely low-frequency magnetic field（50 Hz and 0.3 mT） for 48 hours, and those in the sham-exposure group were also stimulated by horse serum and then exposed to a coil for 48 hours. The NIH3T3 cells were collected, total RNA was extracted, and cDNA was obtained via reverse transcription. Real-time fluorescent quantitative RT-PCR was used to measure the changes in transcription cycles of Cry and Period genes in both groups. Results There was no significant difference in the proliferation rate at 0 , 12, 24, and 48 hours of exposure between the two groups（P〉0.05）. Both sham-exposure group and magnetic field group showed a rhythmic change in the expression of Cry gene, and compared with the sham-exposure group, the magnetic field group had a significantly shortened circadian rhythm of Cry gene in NIH3T3 cells （t=2.57, P〈0.05）. Both groups had rhythmic and periodical expression of Period gene and there was no significant difference between the two groups （t=0.70, P〉0.05）. Conclusion Extremely low-frequency magnetic field can significantly shorten the circadian rhythm of Cry gene in mouse embryonic fibroblasts, while there is no significant change in the circadian rhythm of Period gene.

关 键 词：隐花色素 生物节律 极低频磁场 

分 类 号：R12[医药卫生—环境卫生学]