益气活血散结法对糖尿病肾病大鼠肾组织中MCP-1、TGF-β的影响  

Effects of Qi-tonifying Blood-activating and Mass-dissipating Method on Expressions of MCP-1 And TGF-β in Renal Tissue of Rats with Diabetic Nephropathy

作　　者：黄为钧[1] 王世东[1] 赵进喜[1] 傅强[1] 宫晴[1] 张华[1] 吴文静[2] 申子龙[3] 贾旭[4] 张雨婷[4] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]湖北省中医院,武汉430061 [3]首都医科大学北京中医医院肾病科,北京100010 [4]北京中医药大学科研实验中心,北京100029

出　　处：《世界中医药》2017年第1期16-20,共5页World Chinese Medicine

基　　金：国家自然科学基金项目(编号:81473664)

摘　　要：目的:通过动物实验,基于免疫炎性反应因子MCP-1、TGF-β,探索益气活血散结法改善肾小球硬化的分子机制。方法:24只GK大鼠,随机分为模型组、假手术组、益气活血散结组。通过高脂高能量喂养加单肾切除术复制糖尿病肾病模型。检测各组大鼠的24 h尿蛋白,观察大鼠在干预后肾脏组织的病理改变,同时采用Western blotting以及免疫组化法检测肾脏组织的MCP-1和TGF-β的表达情况,采用RT-PCR法检测MCP-1mRNA以及TGF-βmRNA的表达水平。结果:PASM染色和Masson染色显示:益气活血散结组阳性率明显低于模型组(P<0.01)。益气活血散结组中24 h尿蛋白在药物干预后明显减少(P<0.01)。Western blotting、免疫组化结果均显示:益气活血散结组MCP-1(P<0.05)、TGF-β(P<0.01)的表达程度明显低于模型组。同样,RT-PCR结果也显示:MCP-1mRNA(P<0.01)、TGF-βmRNA(P<0.05)水平在益气活血散结组明显低于模型组。结论:益气活血散结法可以有效地减少24 h尿蛋白定量、延缓肾小球纤维化,其机制可能与抑制肾脏组织中MCP-1和TGF-β的表达有关。Objective：To study the mechanism of Qi-tonifying blood-activating and mass-dissipating method in treating diabetic nephropathy based on MCP-1 and TGF-β through animal experiment.Methods：A total of 24 GK rats were randomly divided into model group,sham-operated group and Qi-tonifying blood-activating and mass-dissipating group.Each group had 8 rats.The DN model was established by feeding with high-fat diet and unilateral nephrectomy.24-hour urine protein was detected and the renal pathological changes were observed.Expressions of MCP-1 and TGF-β in the renal tissue were detected by Western blotting and immunohistochemistry,while RT-PCR was used to detect the expression of MCP-1mRNA and TGF-β mRNA.Results：Results of PASM staining and Masson staining show that the positive rate of Qi-tonifying blood-activating and mass-dissipating group was significantly lower than that of model group（P 〈 0.01）.The 24-hour urinary protein in Qi-tonifying blood-activating and mass-dissipating group decreased significantly after drug intervention（P 〈 0.01）.Results of Western blotting and immunohistochemistry showed that expression levels of MCP-1 （P 〈 0.05） and TGF-β （P 〈 0.01） were lower in Qi-tonifying blood-activating and mass-dissipating group than those in model group.Results of RT-qPCR showed that expression levels of MCP-1 mRNA（P 〈 0.01） and TGF-β mRNA（P 〈0.05） in Qi-tonifying blood-activating and mass-dissipating group were significantly lower than those in model group.Conclusion：The treatment of diabetic nephropathy with Qi-tonifying blood-activating and mass-dissipating method can decrease the proteinuria and delay the glomerular fibrosis.The molecular mechanism may be related to the inhibition of MCP-1 and TGF-β.

关 键 词：MCP-1 TGF-Β 益气活血散结法 糖尿病肾病 

分 类 号：R259[医药卫生—中西医结合]