MMPs和TIMPs与糖尿病心肌病心室重构的关系及糖心宁的干预作用  

MMPs and TIMP's Relationship with Diabetic Cardiomyopathy Ventricular Remodeling and Intervention Effect of Tangxinning

作　　者：谷玉红[1] 李景[1] 王军[2] 解欣然[3] 刘杰[2] 周旭升[1] 岳改英[1] 易京红[1] 魏执真[4] 

机构地区：[1]首都医科大学附属北京中医医院内分泌科,北京100010 [2]首都医科大学基础医学院,北京100069 [3]北京市中医研究所,北京100010 [4]首都医科大学附属北京中医医院心内科,北京100010

出　　处：《世界中医药》2017年第1期25-29,共5页World Chinese Medicine

基　　金：首都中医药研究专项一般项目(编号:15ZY15);首都医科大学附属北京中医医院"育苗计划"院级课题(编号:2014YM-07)

摘　　要：目的:探讨MMP-2、MMP-9、TIMP-2、TIMP-1在糖尿病心肌病心室重构过程中的作用,以及糖心宁的心肌保护作用及机制。方法:36只SD大鼠,其中9只作为空白对照组,27只腹腔注射链脲佐菌素(STZ)制备糖尿病心肌病大鼠模型,随机分为模型组、糖心宁高剂量组、糖心宁等效剂量组3组,糖心宁组灌服中药,模型组及空白对照组灌服等量清洁饮用水,给药8周后处死大鼠,计算左心室肥厚指数,检测血清T-SOD活性和MDA含量;Masson染色观察心肌组织中胶原,免疫组化法检测心肌组织MMP-2、MMP-9、TIMP-2、TIMP-1的蛋白表达。结果:糖尿病心肌病大鼠用药8周后,糖心宁高剂量组、糖心宁等效剂量组均能降低左心室肥厚指数,减少MDA含量,升高T-SOD水平,且同时升高MMP-9/TIMP-1和MMP-2/TIMP-2比值,降低心肌胶原含量。结论:糖心宁具有通过改善氧化应激,重新调整MMP-9/TIMP-1平衡、减弱TIMP-1对MMP-9抑制从而减轻DCM心室重构的作用。Objective：To study the MMP-2,MMP-9,TIMP-2 and TIMP-1＇s function during diabetic cardiomyopathy ventricular remodeling process,as well as the myocardial protective effect and mechanism of Tangxinning.Methods：A total of 36 SD rats were included into the study,9 of them were taken as normal control rats,and the other 27 rats were injected with streptozotocin（STZ）.These 27 rats were randomly divided into three groups：a model group and a high dose group of Tangxinning and an equal dose group of Tangxinning.The groups of Tangxinning were given a gavage of Chinese traditional medicine.The groups of model and control were given a gavage of clean drinking water.The experiment lasted 8 weeks.Then,the rats were killed to calculate the left ventricular hypertrophy index and estimate the activities of the T-SOD and MDA in serum.The cardiac histological changes were observed by Masson staining.The protein expression of MMP-2,MMP-9 and TIMP-2 and TIMP-1 in myocardium were detected by immunohistochemical method.Results：After 8 weeks of medication,both high dose group and the equal dose group of Tangxinning could reduce index of left ventricular hypertrophy and MDA,increase the level of T-SOD and the ratio of MMP-9/TIMP-1 and MMP-2/TIMP-2.The myocardial collagen content dropped.Conclusion：Tangxinning may have the effect to readjust the balance of MMP-9/TIMP-1 by improving oxidative stress and weakening TIMP-1 to MMP-9,Then DCM ventricular remodeling effect was reduced.

关 键 词：糖尿病心肌病 氧化应激 MMPS TIMPS 糖心宁 

分 类 号：R587.2[医药卫生—内分泌]