安神补心胶囊对小鼠大脑内神经递质影响  

Effects of Anshen Buxin Capsule on Neurotransmitter in Cerebral Tissue of Mice

作　　者：陈汉裕 陈凤丽[1] 林赞檬 杨忠奇[2] 

机构地区：[1]广州中医药大学,广州510405 [2]广州中医药大学第一附属医院,广州510405

出　　处：《世界中医药》2017年第1期138-141,共4页World Chinese Medicine

基　　金：国家级大学生创新创业训练计划项目(编号:201410572008)

摘　　要：目的:观察安神补心胶囊对小鼠大脑神经递质5-羟色胺(5-HT)及γ-氨基丁酸(GABA)含量的影响,初步探讨安神补心胶囊改善睡眠作用机制,为临床研究提供实验理论依据。方法:将昆明小鼠随机为空白对照组、地西泮对照组、安神补心胶囊组,分别灌相应药物:0.9%氯化钠注射液、地西泮水溶液(0.1 mg/mL)及安神补心胶囊水溶液(0.1 g药丸/mL),每天灌胃给药1次(0.1 mL/10 g),连续灌胃8 d,末次给药后,用酶联免疫吸附测定法测定小鼠大脑5-HT及GABA含量。结果:空白对照组、地西泮对照组、安神补心胶囊组5-HT含量分别为(604.56±31.35)ng/mL、(468.42±30.33)ng/mL、(518.21±31.20)ng/mL;GABA含量分别为(10.77±1.04)μmol/L、(14.58±0.75)μmol/L(12.07±0.88)μmol/L。与空白对照组相比,安神补心胶囊组能明显降低小鼠大脑5-HT含量及提高小鼠大脑GABA含量,差异有统计学意义(P<0.05),与地西泮对照组比较,差异亦具有统计学意义(P<0.05)。结论:安神补心胶囊具有良好的改善睡眠作用,其作用机制可能与抑制减少大脑5-HT的合成及刺激增加大脑GABA的分泌有关。Objective：To study the effect of Anshen Buxin capsule on serotonin （5-HT） and γ-aminobutyric acid content in cerebral tissue of mice,so as to make a preliminary discussion on its mechanism of improving sleep and then experimental theory for clinical study.Methods：Kunming mice were randomly divided into 3 groups,and each group was filled the corresponding drugs intragastrically：normal saline,aqueous solution of diazepam （0.1 mg/mL） and Anshen Buxin capsule （0.1 g/mL）.All of those were given once a day （0.1 mL/10 g） and lasted for 8 days.After the last day of drug administration,use ELASA method to detect the content of 5-HT and GABA in mice brain serum.Results：The contents of 5-HT and γ-aminobutyric acid in normal,diazepam and Anshen Buxin capsule group were （604.56 ± 31.35） ng/mL,（10.77 ± 1.04） μmol/L,（468.42 ± 30.33） ng/mL,（14.58 ± 0.75） μmol/L;（518.21 ± 31.20） ng/mL,and （12.07 ± 0.88） μmol/L.Compared with normal saline group,the 5-HT content of Anshen Buxin capsule group reduced and GABA content in cerebral tissue of mice increased with significant differences （P 〈 0.05）.Besides,compared with diazepam control group,significant difference also exists （P 〈 0.05）.Conclusion：Anshen Buxin capsule had a sound effect in improving sleep quality,and its mechanism may be related to inhibit the production of 5-HT and stimulate GABA secretion in brain.

关 键 词：安神补心胶囊 神经递质 睡眠 机制 

分 类 号：R285.5[医药卫生—中药学]