哈尔滨市儿童肠道病毒71型中和抗体水平调查研究  

Investigation on the neutralizing antibody level of enterovirus type 71 of children in Harbin

作　　者：范荣军[1,2] 曹家铭[2] 崔国权[1] 赵志磊[2] 姜春来[2] 梁英[1] 

机构地区：[1]哈尔滨市疾病预防控制中心,黑龙江哈尔滨150056 [2]吉林大学生命科学学院,吉林长春130012

出　　处：《中国卫生检验杂志》2017年第14期2095-2097,共3页Chinese Journal of Health Laboratory Technology

基　　金：哈尔滨市应用技术研究与开发项目(2014RFXGJ064)

摘　　要：目的了解哈尔滨市0岁~14岁不同人群儿童肠道病毒71型(enterovirus 71,EV71)中和抗体(neutralizing antibody,NA)水平,为防控EV71引起的手足口病流行提供科学依据,为手足口病疫苗效果评价提供基础数据。方法选取哈尔滨市4家医院作为样品采集点,采集临床排除手足口病者的样本413份,应用假病毒系统检测样本EV71中和抗体滴度,样本率的比较用χ~2检验。结果 413份样本EV71中和抗体阳性率为37.05%;不同性别、时间EV71阳性率差异均无统计学意义(P>0.05);不同年龄组儿童EV71阳性率不同,差异有统计学意义(χ~2=19.63,P<0.05)。其中≤6个月组EV71中和抗体阳性率最高,为68.00%;7个月~12个月组EV71中和抗体阳性率最低,为20.83%。结论中和抗体较低的人群及时间点为手足口病防治的重点。Objective To investigate the level of anti-enterovirus 71（ EV71） neutralizing antibody（ NA） in children aged 0-14 years in Harbin,so as to provide scientific basis for the prevention of hand,foot and mouth disease caused by EV71,and basic data for the evaluation of the hand-foot-mouth vaccines effectiveness. Methods Four hospitals in Harbin were selected as sample collection sites,and 413 samples were collected from patients with hand,foot and mouth disease. The anti-EV71 NA titer was detected using pseudovirus system. χ~2-test was used to compare the sample rate. Results The positive rate of the anti-EV71 NA was 37. 05% in 413 samples. The differences of the positive rate of anti-EV71 NA in different gender groups or in different collection time had no statistical significance（ P〈0. 05）. The differences of the positive rate of anti-EV71 NA in different age groups had statistical significance（ χ2= 19. 63,P〈0. 05）. The positive rate of anti-EV71 NA was the highest in children younger than 6 months（ 68. 00%）,and the positive rate of anti-EV71 NA was the lowest in 7 to 12 months children（ 20. 83%）. Conclusion The prevention of hand,foot and mouth disease should focus on the population who have lower level of anti-EV71 NA and the time in which the level of anti-EV71 NA is low.

关 键 词：哈尔滨市 肠道病毒EV71型 中和抗体 水平 假病毒 调查研究 

分 类 号：R373.25[医药卫生—病原生物学]