益肺活血颗粒对香烟提取物诱导的巨噬细胞NF-κB活性的影响  

Effect of Yifei Huoxue Granules on Activity of NF-κB in PMA-differentiated U937 Cells after Stimulation of Cigarette Smoke Extract

作　　者：简芙平 欧敏[1] 

机构地区：[1]海军总医院干部呼吸科,北京100048

出　　处：《解放军药学学报》2017年第3期203-206,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：军队中医药科研专项课题基金项目;No.10ZYZ219

摘　　要：目的探讨益肺活血颗粒对香烟提取物诱导的巨噬细胞核转录因子-κB(NF-κB)活性的影响。方法用12-豆蔻酸-13-乙酸佛波醇将U937诱导为巨噬细胞,0.6~9.6g·L^(-1)的益肺活血颗粒及0.5%~10%的香烟提取物处理巨噬细胞,于12、24、48h细胞活力检测法测定细胞活性;再将巨噬细胞分为对照组、香烟提取物组、香烟提取物+益肺活血颗粒组、香烟提取物+小白菊内酯组,分别于12、24、48h采用免疫印迹法检测细胞内NF-κB p50/p65/p-IκB表达水平。结果香烟提取物能够诱导NF-κB p50/p65/p-IκB的蛋白表达,而益肺活血颗粒和小白菊内脂能抑制香烟提取物对p50/p65/p-IκB表达的诱导作用(P<0.05),但小白菊内脂抑制作用强于益肺活血颗粒(P<0.05)。结论益肺活血颗粒能够抑制香烟提取物诱导的巨噬细胞NF-κB的活化。Objective To investigate the effect ofYifei Huoxue granules on activity of nuclear factor-κB in mac- rophages after stimulation of cigarette smoke extract （CSE）.Methods The U937 monocytic cells were differentiated into macrophages using phorbol 12-myristate 13-acetate （PMA）. These macrophages were treated with Yifei Huoxue gran- ules 0.6--9.6 g·L^-1 or 0.5%--10% CSE.At the 12, 24 and 48 h time point, the cells-viability was assessed using the cell counting kit-8 （CCK-8）. Moreover,macrophages were randomly divided into the control group, the CSE group, the CSEq- Yifei Huoxue granules group, and the CSE＋parthenolide group. Expression of p50/p65/p-IκB was exam- ined by western blot at 12, 24, 48 h time points.Results CSE could induce the protein expression of p50/p65/p-κB in macrophages. Yifei Huoxue granules and parthenolide had inhibitory effect on activation of NF-κB induced by CSE in macrophages （ P 〈0.05）. However, the p50/p65/ p-IκB protein expression in CSE＋parthenolide group was lower than that in CSE＋ Yifei Huoxue granules group （ P %0.05）.ConclusionYifei Huoxue granules can inhibit the activa- tion of NF-κB induced by CSE in macrophages.

关 键 词：益肺活血颗粒 巨噬细胞 核转录因子-ΚB 小白菊内酯 

分 类 号：R969.4[医药卫生—药理学]