槲皮素改善胰岛素抵抗HepG2细胞糖代谢的研究  

Study on the Improving Effect of Quercetin on Glucose Metabolism in Insulin Resistant HepG2 Cells

作　　者：彭晶[1] 郑锦坤[1] 何蓉蓉[1] 彭玉梅[1] 李庆德[1] 

机构地区：[1]粤北人民医院

出　　处：《今日药学》2017年第7期436-440,共5页Pharmacy Today

基　　金：广东省医院药学研究基金(2016A02);广东省医学科学技术研究基金(A2016591)

摘　　要：目的研究槲皮素对棕榈酸(PA)诱导的胰岛素抵抗HepG2细胞糖代谢的改善作用。方法用棕榈酸诱导的HepG2细胞建立胰岛素抵抗模型。用MTT法观察不同浓度槲皮素对HepG2细胞的影响,用葡萄糖氧化酶法检测葡萄糖消耗,用实时PCR(Real-time PCR)的方法检测AKT、GSK-3β、G6Pase、GCK的基因表达,用免疫印迹法(Western blotting)检测AKT、GSK-3β的磷酸化蛋白表达及G6Pase、GCK的蛋白表达。结果槲皮素能够增加棕榈酸诱导HepG2细胞中的葡萄糖消耗,明显增加棕榈酸诱导HepG2细胞中AKT的磷酸化水平,降低GSK-3β磷酸化水平,降低G6Pase基因和蛋白表达,增加GCK基因和蛋白表达。结论槲皮素能改善棕榈酸诱导的胰岛素抵抗HepG2细胞的糖代谢作用,与其激活AKT信号通路密切相关。OBJECTIVE To investigate the improving effect of quercetin on glucose metabolism in PA-induced insulin resistant HepG2 cells. METHODS HepG2 cells were treated by PA to induce insulin resistance model. MTT assay was used to observe the influence of different concentration of quercetin on HepG2 cells. Glucose oxidation method was used to measure glucose consumption.Real-time PCR assay was used to detect the RNA expression levels of AKT,GSK-3β,G6 Pase and GCK. Western blotting assay was used to detect the phosphorylation levels of AKT and GSK-3β,and the protein levels of G6 Pase and GCK. RESULTS Quercetin effectively increased glucose consumption in PA-induced HepG2 cells. Quercetin markedly increased the phosphorylation levels of AKT,mRNA and protein levels of GCK,decreased the phosphorylation levels of GSK-3β,mRNA and protein levels of G6 Pase in PA-induced HepG2 cells.CONCLUSION The study confirmed that the improving effect of quercetin on glucose metabolism in PA-induced insulin resistant HepG2 cells is closely relevant to its role in activating AKT pathway.

关 键 词：槲皮素 胰岛素抵抗 AKT 

分 类 号：R965[医药卫生—药理学]