高效液相色谱法测定柴芍胃炎颗粒中芍药苷的含量  

Content Determination of Paeoniflorin in Chaishao Gastritis Granules by HPLC

作　　者：袁常珍 梅刚 

机构地区：[1]泸州市食品药品检验所,四川泸州646000 [2]泸州市泸县中医医院药剂科,四川泸州646000

出　　处：《中国医院用药评价与分析》2017年第7期938-939,943,共3页Evaluation and Analysis of Drug-use in Hospitals of China

摘　　要：目的:采用高效液相色谱法测定柴芍胃炎颗粒中芍药苷含量。方法:色谱柱为ZORBAX SB-C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液(V∶V=22∶78),检测波长为230 nm,流速为1.0 ml/min,柱温为30℃。结果:芍药苷的质量浓度在49.68~186.3μg/ml范围内与峰面积呈良好线性关系(r=0.999 7,n=5),芍药苷质量浓度的平均加样回收率为96.05%,RSD为0.56%(n=6)。结论:本方法简便、灵敏、检测结果准确,可用于柴芍胃炎颗粒的质量控制。OBJECTIVE： To establish a method for content determination of paeoniflorin in Chaishao gastritis granules by HPLC. METHODS： The column was ZORBAX SB-C_（18）column（ 250 mm × 4. 6 mm,5 μm）,the mobile phase consist of acetonitrile-0. 1% phosphoric acid solution（ V ∶ V = 22 ∶ 78）,with detective wave length of 230 nm,flow rate of 1. 0 ml/min and column temperature of 30 ℃. RESULTS： The calibration curve of paeoniflorin was linear over the range of 49. 68 μg/ml-186. 3 μg/ml（ r = 0. 999 7,n = 5）. The average recovery rate was 95. 05%,RSD was0. 56%（ n = 6）. CONCLUSIONS： This method is simple,sensitive,accurate and reliable,and can be used for the quality control of Chaishao gastritis granules.

关 键 词：高效液相色谱法 柴芍胃炎颗粒 芍药苷 含量测定 

分 类 号：R927.2[医药卫生—药学]