光合作用合成相关差异蛋白对应基因在高油酸油菜近等基因系中表达规律研究  

Expression Patterns of Genes according to Photosynthesis-related Differential Proteins in High Oleic Acid Rapeseed Isogenic Lines

作　　者：王晓丹[1] 肖钢[1] 张振乾[1] 官春云[1] 陈社员[1] 邬贤梦[1] 张诗远 WANG Xiao-dan XIAO Gang ZHANG Zhen-qian GUAN Chun-yun CHEN She-yuan WU Xian-meng ZHANG Shi-yuan(College of Agriculture, Hunan Agricultural University/Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China Hunan Changsha 410128, China)

机构地区：[1]湖南农业大学农学院/南方粮油作物协同创新中心,湖南长沙410128

出　　处：《西南农业学报》2017年第7期1483-1487,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金"基于蛋白组学的油菜高油酸关键基因鉴定研究"(31201240);国家973计划"主要环境因子及生育期影响油菜油脂积累的分子基础"(2015CB150206);国家科技支撑计划"油菜商业化育种研究与模式示范"(2014BAD01B07);湖南省科技重大专项"高油酸油菜产业化发展关键技术研究与示范"(2014FJ1006);作物种质创新与资源利用国家重点实验室培育基地开放课题"与脂肪酸合成相关基因表达量研究及其功能验证";湖南农业大学作物学开放基金"高油酸油菜近等基因系差异基因研究"(ZWKF201302)

摘　　要：【目的】本文探索了光合作用相关基因表达与蛋白表达之间的关系,加速高油酸油菜育种进程。【方法】以高油酸油菜近等基因系出苗后7 d、5~6叶期和越冬期的叶片、花瓣和授粉后20~35 d的种子及角果皮为材料,利用定量PCR方法研究9个i TRAQ分析得到的差异蛋白对应基因在不同生育期表达规律。【结果】在出苗后7 d、花期和授粉后20~35 d,高油酸油菜的光合作用能力低于低油酸油菜;5~6叶期、越冬期高油酸油菜的光合作用能力高于低油酸油菜。gi|515616基因在高油酸油菜出苗后7d去根材料及低油酸油菜5~6叶期、越冬期叶片和授粉后20~35 d角果皮中不表达,在花、授粉后20~35 d种子中均不表达。gi|297853098,gi|297825149和gi|312282567基因及gi|50313237和gi|79475768基因分别具有相同的表达规律。gi|297825149基因在高油酸油菜角果皮中是唯一上调表达基因。gi|312282897基因在高油酸油菜出苗后7 d去根材料为唯一上调表达基因。【结论】这些基因可作为高油酸油菜育种材料筛选的参考基因,加快育种进程。[ Objective] In this paper, the relationship between gene expression and protein expression in photosynthesis were discovered so that the process of high oleic acid rape breeding could be accelerated. [ Method] The 7 days after emergence, 5 - 6 leaf stage, leaves of win- tering period, petals, seeds and pods skins 20 - 35 days after pollination of high oleie acid rapeseed isogenic lines were used as tested mate- rials to find out the expression rules in different development periods. The nine corresponding genes of the differential proteins which derived from the iTRAQ analysis were studied by Real-time quantitative PCR. [ Result] In the development of 7 days after emergence, flowering and pollination 20 - 35 days, high oleic acid rapeseed had lower photosynthesis ability than low oleic acid rapeseed, while in the stages of 5 - 6 leaf and over-wintering, high oleic acid rapeseed had higher photosynthesis ability than low oleic acid rapeseed. Gi 1515616 gene did not exist in high oleic acid rape of 7 days after emergence, the leaves of 5 -6 leaf stage and over-wintering stage, pod skins 20 -35 days after pollination of low oleic acid rapeseed, flower and seed 20 - 35 days after pollination of the two lines. The genes of gi 1297853098, gi 1297825149 and gi 1312282567 had the same expression rules, while g1150313237 and gi 179475758 had the same expression rules too. Gi 1297825149 and gi 1312282897 were the only up-regulated gene in pods skins and 7 days after emergence of high oleic acid rapeseed respectively. [ Conclusion] These genes could be used as reference genes to screen high oleic acid rapeseed breeding materials, which would be useful to speed up the breeding process.

关 键 词：实时荧光定量PCR 光合作用 高油酸油菜 

分 类 号：S565[农业科学—作物学]