魔芋软腐病菌血清学检测方法的建立  

Establishment of Serological Detection Method against Soft Rot Pathogen of Amorphophallus konjac

作　　者：张小芳[1] 张海燕[1] 王永吉[1] 魏兰芳[2] 姬广海[1] ZHANG Xiao-fang ZHANG Hai-yan WANG Yong-ji WEI Lan-fang JI Guang-hai(College of Plant Protection, Yunnan Agrieuhural University, Yunnan Kunnfing 650201 , China Agrieultural Foundation Experiment Teaching Center, Yunnan Agricultural University, Yunnan Kunming 650201, China)

机构地区：[1]云南农业大学植物保护学院,云南昆明650201 [2]云南农业大学农科基础实验教学中心,云南昆明650201

出　　处：《西南农业学报》2017年第7期1576-1581,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学地区基金(31360002;31460458)

摘　　要：【目的】魔芋软腐病是魔芋生产中的一个重要细菌性病害,严重威胁着魔芋产量。【方法】本实验拟利用血清学技术建立快速检测魔芋细菌性软腐病菌的方法。【结果】采用棋盘滴定法确定抗原和抗血清的最适工作浓度分别为106CFU/m L和1∶10000;病原菌检测灵敏度为103CFU/m L;抗血清与其它细菌标准菌株的交叉反应结果均呈阴性,结果表明该方法具有较高的特异性。将该方法检测了24份魔芋种球、病土和带病植株,检出率高达91.67%。【结论】该技术可以有效的检测魔芋软腐病原菌胡萝卜软腐果胶杆菌(Pectobacterium carotovora subsp.carotovora,p.c.c.),为生产上魔芋种球快速检测软腐病菌提供了方法。[ Objective] The soft rot of Amorphophallus konjac（A, konjac） is the major disease affecting the yield of A. konjac. [ Method ] In this study, a method for rapid detection of the soft rot pathogen of Amorphophallus konjac was established by serological technique. [ Result ] Using checkerboard titration, the best working concentration of antigen and antiserum were 106 CFU/mL and 1 ： 10000 respeefively. The sen- sitivity of the serum was tested, and the lowest p. c. c. suspension was 103 CFU/mL. Cross reactions of antisera with other bacteria were de- tected, all results were negative. The cross assay indicated that this method had high serological specificity. The method has been used to de- tect 24 samples, with the detective rate 91.67 %. [ Conclusion]The results indicated that this method can be used to detect the pathogens of p. c. c.. This is very useful for diagnosing latent infection ofp. c. c of seed tubers in the early stage.

关 键 词：魔芋软腐病 细菌性病害 血清学检测 

分 类 号：S436.32[农业科学—农业昆虫与害虫防治]