荔波瑶山鸡POU1F1基因多态性与繁殖性状的相关性  

Association of Polymorphic Sites of Gene POU1F1 with Chicken Egg Laying Traits

作　　者：朱丽莉[1] 陶宇航[1] 韩雪[1] 唐继高[1] 吴松成[1] 李亮[1] 伍革民[1] ZHU Li-li TAO Yu-hang HAN Xue TANG Ji-gao WU Song-cheng LI Liang WU Ge-min(Guizhou Institute of Animal Husbandry and Veterinary Science, Guizhou Guiyang 550005, Chin)

机构地区：[1]贵州省畜牧兽医研究所,贵州贵阳550005

出　　处：《西南农业学报》2017年第7期1675-1679,共5页Southwest China Journal of Agricultural Sciences

基　　金：贵州省联合基金项目"瑶山鸡ONECUT1基因的CDS区克隆;差异表达及与生产性状关联分析"[黔科合LH字(2015)7067];贵州省基金项目"瑶山鸡就巢性选育关键基因筛选"[黔科合J字(2014)2102];贵州省农业动植物育种项目"瑶山鸡新品系选育与推广"[黔农育专字(2014)008];贵州省农业科学院2014年博士基金项目"瑶山鸡STAT5基因变异检测及与产蛋性状关联分析";贵州省农业科学院院专项"长顺绿壳蛋鸡绿壳表型遗传标记的筛选与应用"[黔农科院院专项(2014)006];"百宜黑鸡高产纯繁核心种群选育与示范推广"[黔农科院院专项(2014)038];市院地合作项目"喀斯特地形山地肉鸡养殖技术推广"[院地农科合字(2014)10]

摘　　要：【目的】为探明荔波瑶山鸡POU1F1基因多态性与繁殖性状的关系,为荔波瑶山鸡的选择性育种提供参考。【方法】针对POU1F1基因设计6对引物,采用PCR测序法筛选出多态位点,并利用荔波瑶山鸡母系60个个体进行繁殖性状的关联分析。【结果】荔波瑶山鸡POU1F1基因有8个多态位点,其中POU1F1基因58(A→G)与300日龄产蛋量、首次就巢日龄、300日龄就巢次数均呈显著或极显著相关(P<0.05或P<0.01);65(T→C)与300日龄产蛋量、首次就巢日龄、300日龄就巢次数呈显著相关(P<0.05);11041(T→C)与300日龄产蛋量、首次就巢持续时间、首次就巢至重新开产间隔均呈显著或极显著相关(P<0.05或P<0.01)。【结论】POU1F1基因58(A→G)和65(T→C)可作为300日龄产蛋量、首次就巢日龄和300日龄就巢次数的辅助选择标记;11041(T→C)可作为300日龄产蛋量、首次就巢持续时间、首次就巢至重新开产间隔的辅助选择标记。[ Objective] The stydy aims to analyze the associations of gene POUIF1 with chicken egg laying traits and provide references for selection breeding of Libo Yaoshan chicken. [ Method] Six primers were designed and eight polymorphic sites were selected, and the association analysis was performed in 60 chickens of Libo Yaoshan female line by PCR-sequencing methods. [ Result ] 58 （A-→G） mutation sites were significantly associated with the total egg number from 120 to 300 d of age （EN300）, age at first broodiness behavior （AFBB） and times of broodiness behavior from 120 to 300 d of age （TBB300） （P 〈 0.05 or P 〈 0. 01 ）. 65 （T→C） mutation sites were significantly associated with EN300, AFBB and TBB300 （ P 〈 0.05 ）. 11041 （T→C） mutation sites were significantly associated with EN300. Duration of age at first broodiness behavior （DAFBB） and interval of age at first broodiness behavior between anew laying （IAFBBA） （ P 〈 0.05 or P 〈 0. 01 ）. [ Conclusion ] 58 （A→G） and 65 （T→C） may be regarded as an effective molecular marker on the MAS（ Marker Assisted Selection） program for EN300, AFBB and TBB300; 11041 （T→C） may be regarded as an effective molecular marker for EN300, DAFBB and IAFB-A.

关 键 词：POU1F1基因 基因多态位点 荔波瑶山鸡 繁殖性状 

分 类 号：S831[农业科学—畜牧学]