1株分离自矽肺合并肺部感染患者痰液中Empedobacter falsenii 5-1的鉴定  

Identification of Empedobacter falsenii 5-1 from the sputum of patients with silicosis complicated with pulmonary infection

作　　者：陈春兰[1] 张建华[1] 张靖[1] 徐东伟[2] 尚慧[1] 兰晓君[2] Chen Chunlan Zhang Jianhua Zhang Jing Xu Dongwei Shang hui Lan Xiaojun(Jinchuan Group Workers Hospital, Jinchang 737101, China Jinchuan Industrial Hygiene Institute of Nickel ＆ Cobalt, Jinchang 737103, China)

机构地区：[1]金川集团公司医院,甘肃金昌737101 [2]金川集团公司镍钴工业卫生研究所,甘肃金昌737103

出　　处：《甘肃医药》2017年第7期528-531,共4页Gansu Medical Journal

基　　金：金川集团有限公司科技部项目(项目编号:金科综2010-08)

摘　　要：从1名确诊为矽肺合并肺部感染患者痰液中分离出1株革兰氏染色阴性的菌株5-1,细胞杆状,无运动,无鞭毛,生长温度范围为15~40℃,最适生长温度为37℃,生长的p H范围是6~9。菌株5-1与Empedobacter falsenii NF 993T的16S r RNA相似性为98.49%,系统发育分析显示,该菌株与Empedobacter falsenii NF 993T在同一个分支,亲缘关系最近。细胞壁脂肪酸主要是iso-C15:0,iso-C17:03-OH和Sum In Feature 3(C16:1ω7c/C16:1ω6c);主要的呼吸醌是甲基萘醌6;DNA的G+C百分含量为33.55%。综合系统发育、表型特征和化学特征,菌株5-1被鉴定为Empedobacter falsenii。A yellow-coloured strain,designated 5-1 ,was isolated from the sputum of patients with silicosis complicated with pulmonary infection. The cells were rod-shaped,gram-negative and no motile. Growth occurred at 15～40℃ （optimum, 37℃ ） and pH 6-9 （optimum,pH 7.0）. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolate formed a cluster with species Empedobacter falsenii in the genus Empedobacter. Sequence similarities with the type strains of recognized species ranged from 90.65% to 98.49%. The predominant respiratory quinone was MK-6 and the major cellular fatty acids were iso-C15：0,iso-C17：0 3-OH, summed feature 3 （containing C16：1ω7c and/or C16：1ω6c ）. The DNA G＋C content was 33.55%. On the basis of the phylogenetic, phenotypic, and chemotaxonomic data, strain 5-1 was identified as Empedobacterfalsenii.

关 键 词：矽肺 肺部感染 Empedobacter falsenii5-1 鉴定 

分 类 号：R446.5[医药卫生—诊断学]