肠炎沙门菌PFGE分子分型及耐药性研究  

Pulsed-field gel electrophoresis （PFGE）molecular subtyping and drug resistance of Salmonella enteritidiss trains isolated in Longyan city

作　　者：陈前进[1,2] 曹春远[1] 李美华[1] 陈海滨[1] 廖琳虹[1] 何春荣[1,2] 金建潮[1] 

机构地区：[1]龙岩市疾病预防控制中心,364000 [2]福建医科大学龙岩市疾病预防控制中心教学点,364000

出　　处：《中华微生物学和免疫学杂志》2017年第7期534-539,共6页Chinese Journal of Microbiology and Immunology

基　　金：福建省卫生厅青年科研课题项目（2014-1-96）;2014年龙岩市第二批科技项目（2014LY29）

摘　　要：目的分析龙岩市肠炎沙门菌脉冲场凝胶电泳（PFGE）分子分型特点和耐药特征，为食源性疾病暴发的发现及疫情控制和临床诊治提供参考依据。方法对收集自2014、2016年龙岩市食物中毒、食品安全风险监测和临床散发病例分离的77株肠炎沙门菌进行PFGE分析，并测定其中57株对16种常用药物的最小抑菌浓度（minimal inhibitory concentration，MIC）。结果77株肠炎沙门菌分为17个PFGE型别，其中P4、P6和P8为优势型别；按照90％的相似度可得到3个优势PFGE型别簇（Cluster 1—3）。暴发相关的带型主要为P8、P6、P4和P14，分别与5次食物中毒事件有关。57株肠炎沙门菌药敏结果以耐萘啶酸最高（93％），其次是氨苄西林（74％）和链霉素（54％）。耐3种或3种以上抗生素的多重耐药菌占71．93％（41／57）。结论龙岩市肠炎沙门菌PFGE型别呈现多态性，存在3个优势PFGE型别簇，在食物中毒事件中以PFGE分子分型检测能快速预警肠炎沙门菌散在暴发和溯源。肠炎沙门菌多重耐药现象应引起重视，需加强对多重耐药株的监控及抗菌药物使用的监管。Objective To analyze the pulse-field gel electrophoresis （PFGE） patterns and antibi- otic resistance of Salmonella enteritidisstrains isolated in Longyan city in order to provide reference for effective control, clinical diagnosis and treatment of outbreaks of food-borne diseases. Methods Seventy-seven Salmonella enteritidis strains isolated from food poisoning cases, livestock and poultry meat samples collected for food safety risk monitoring and sporadic cases in 2014 and 2016 were analyzed by PFGE. Antibiotic sus- ceptibility testing was carried out to determine the minimum inhibitory concentrations （MIC） of 16 common antibiotics to 57 strains. Results Seventeen PFGE types were identified among the 77 Salmonella strains including three predominant types （ P4, P6 and P8 ） and were grouped into three major pulsotypes （ Clusterl- 3 ） at 90% similarity level. The predominant types causing outbreaks of Salmonella enteritidis infection were P8, P6, P4 and P14, which were responsible for five cases of food poisoning. These isolated strains were highly resistant to nalidixic acid （93%） , followed by resistance towards ampicillin （74%） and streptomycin （54%）. Forty-one strains were resistant to three or more antibiotics （71.93%）. Conclusion The PFGE types of Salmonella enteritidis strains isolated in Longyan city exhibit polymorphism and are clustered into three major pulsotypes. PFGE molecular typing can provide early-warning of sporadic outbreaks and help to identify the sources of infections in Salmonella enteritidis-related food poisoning. Attentions should be paid to muhidrug resistant Salmonella enteritidis. Surveillance for multidrng resistant strains and usage of antibiotics should be strengthened.

关 键 词：肠炎沙门菌 PFGE分子分型 聚类分析 最小抑菌浓度 多重耐药 

分 类 号：R446.5[医药卫生—诊断学]