基于GBS构建玉米高密度遗传图谱及营养品质性状QTL定位  

Construction of High Density Genetic Map via GBS Technology and QTL Mapping for Nutritional Quality Traits in Maize(Zea mays)

作　　者：赖国荣[1] 张静 刘函[2] 董小妹[2] 赖锦盛[2] 黄亚群[1] 陈景堂[1] 郭晋杰[1] 

机构地区：[1]河北农业大学农学院/国家玉米改良中心河北分中心/河北省作物种质资源实验室,保定071000 [2]中国农业大学/农业生物技术国家重点实验室/国家玉米改良中心,北京100193

出　　处：《农业生物技术学报》2017年第9期1400-1410,共11页Journal of Agricultural Biotechnology

基　　金：河北省现代农业技术体系玉米创新团队项目(No.HBCT2013020204);河北省科技计划项目(No.14226305D-5;No.16226323D-2)

摘　　要：高密度遗传连锁图谱的构建可以为玉米遗传作图研究提高定位精度。本研究以玉米自交系X178和NX531为亲本,构建了包含150个株系的重组自交系(recombination inbred line,RIL)群体。基于测序的基因分型技术(genotyping-by-sequencing,GBS)获得基因型纯合的多态性SNP(single nucleotide polymorphism)位点,使用滑动窗口(sliding windows)的方法进行基因型分型,构建了总长为2 569 c M的高密度遗传图谱,标记间平均遗传距离为0.35 c M。为了验证图谱定位可靠性,结合RIL群体籽粒脂肪含量、赖氨酸含量、蛋白质含量和淀粉含量4个营养品质性状的表型数据,使用R/qtl软件包及复合区间作图模型进行QTL定位,共检测到20个营养品质性状相关QTL。本研究所构建的高密度遗传连锁图谱将为玉米农艺性状的遗传基础研究提供良好的基础,同时为玉米籽粒品质的育种改良提供一定的参考。Molecular genetics and breeding of maize have been promoted and developed more efficiently, during the further study of maize genomics. The rapid development of the second generation sequencing technology possesses the characteristics of high throughput and low cost. Nowadays, more and more researchers began to conduct the population size of re-sequencing work, which promoted the analysis of maize genotype and the mining information of genetic variation. In this study, we constructed a recombination inbred line （RIL） mapping population crossed from two elite inbred lines of maize, X178 and NX531, consisting of 150 individuals. The library building and high-throughput sequencing of RIL population were conducted by genotype by sequencing method （GBS）. Besides, the depth re-sequencing was applied for the two parents of the recombinant inbred lines, and the high throughput SNP markers of the populations were obtained. Using a sliding window method, the genotyping of the RIL population was carried out, and then the genetic linkage map was constructed based on bin markers. In order to verify the reliability of this map, the R/ qtl packages and composite interval mapping （CIM） model was used for the mapping of QTLs responsible for nutritional quality traits of maize kernel. 5.0 x sequencing of the parents X178 and NX531 was performed, 881 259 high quality SNPs was obtained and there were polymorphisms between the parents. Based on the SNP between parents, a total of 1 842 109 SNP not only showed genotype homozygosity but also had the same genotype of one parental in the 150 lines of the RIL population. The minimum number of the SNP in one inbred line was 1 673, while the maximum number of the SNPs in one inbred lines was 20 403 and the mean SNP number in every inbred lines was 12, 281. After the screening, 124 RIL strain were chosen for further genotyping and the bin map containing 7 278 recombination bin were obtained. The maximum physical distance between two adjacent bins was 3.28 Mb, and the minimum phys

关 键 词：玉米(Zea mays) 基于测序的基因分型技术 高密度遗传图谱 营养品质性状 数量性状位点 

分 类 号：S513[农业科学—作物学]