人参皂苷Rg1热裂解产物对H_(22)荷瘤小鼠的抗肿瘤作用  

Anti-tumor Effect of Ginsenoside Rg1 Pyrolysis Products (HPPRg1) on H_(22) Tumor-bearing Mice

作　　者：王梓[1] 许兴月 李琼[1] 蔡恩博[1] 李月茹[1] 李伟[1] 张连学[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《中国药学杂志》2017年第15期1319-1324,共6页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(31201331);吉林省留学归国人员启动基金人参产业技术体系资助(201303111);吉林省留学回国人员科研启动基金资助;吉林农业大学首批青年拔尖创新人才支持计划资助

摘　　要：目的探讨人参皂苷Rg1热裂解产物(HPPRg1)对H_(22)荷瘤小鼠的抗肿瘤作用以及机制。方法建立ICR小鼠H_(22)皮下移植瘤小鼠模型,观察HPPRg1的抗肿瘤作用。首先将H_(22)荷瘤小鼠随机分为空白组(normal)、模型组(model)、阳性药环磷酰胺组(CTX,30 mg·kg^(-1))、HPPRg1低、中和高给药组(10、20和40 mg·kg^(-1))。通过计算抑瘤率、脏器指数和肿瘤组织切片染色来观察HPPRg1的抗肿瘤作用。结果与模型组比较,HPPRg1 3个剂量组均能剂量依赖性抑制H_(22)肿瘤的增长(r=0.99,P<0.05),抑瘤率分别为35.7%,42.9%和47.3%;此外,HPPRg1能够不同程度升高小鼠血清中肿瘤坏死因子(TNF)-α,干扰素(IFN)-γ和白细胞介素(IL)-2水平,病理组织染色表明,HPPRg1能够明显促进肿瘤细胞凋亡和坏死;其主要通过抑制瘤重细胞增殖及促凋亡而发挥抗肿瘤作用。结论 HPPRg1对H_(22)移植瘤具有明显的抑制作用,其机制可能与促进肿瘤细胞凋亡及提高机体免疫力有关。OBJECTIVE To investigate the antitumor effect and molecular mechanism of ginsenoside Rgl pyrolysis products （ HPPRgl ） on H22 tumor bearing mice. METHODS To establish tumor model of transplanting H22 tumor-bearing mice and observe the anti-tumor effects of HPPRgl, H22 tumor-bearing mice were randomly divided into groups of control, model, cyelophosphamide （CTX, 30 mg kg-1 ）, low dosage of HPPRgl （HPPRgl-L, 10 mg kg-1 ）, middle dosage of HPPRgl （HPPRgl-H, 20 mg kg-1 ） and high dosage of HPPRgl （ HPPRgl-H, 40 mg kg - 1 ） groups, respectively. Through evaluating inhibition rates of tumors, organ indiees, and levels of TNF-α, IFN-γ and IL-2 to observe the anti-tumor effect of HPPRgl. In addition, H＆E and Hoechst 33258 strai- ning were used to observe the apoptosis of H22 tumor cell. RESULTS Compared with the model group, the three dose groups of HP- PRgl can inhibit tumor proliferation. Mainly through the inhibition of tumor cell proliferation and pro-apoptosis to exert anti-tumor effect. CONCLUSION HPPRgl has a significantly inhibitory effect on H22 tumor-bearing mice, the mechanism may related to pro- mote apoptosis of tumor cells and improve immunity.

关 键 词：人参皂苷RG1 热裂解产物 H22荷瘤小鼠 抗肿瘤 

分 类 号：R965[医药卫生—药理学]