太原市冬夏季PM_(2.5)亚慢性染毒对大鼠肺炎症因子和CK-18表达的影响  

Effects of Subchronic Exposure of Ambient Fine Particulate Matter in Winter and Summer in Taiyuan on Gene Expressions of Cytokines and Cytokeratin 18 in Lungs of Rats

作　　者：李瑞金[1] 张玫[1] 赵利芳[1] 王影[1] 苏瑞军[1] 刘小娜[1] 李卓玉[1] 

机构地区：[1]山西大学环境科学研究所生物技术研究所,山西太原030006

出　　处：《山西大学学报（自然科学版）》2017年第3期622-627,共6页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(91543202)

摘　　要：研究采集PM_(2.5)样本,制备PM_(2.5)生理盐水混悬液,对雄性SD大鼠进行染毒实验,检测大鼠肺组织病理学改变,测定肺中炎症因子和CK-18mRNA和蛋白表达。结果显示,随着PM_(2.5)染毒剂量增加,肺组织出现肺充血和炎性细胞浸润等炎症反应,冬季PM_(2.5)所致肺病理学损伤比夏季严重。冬季PM_(2.5)中、高剂量组和夏季高剂量组大鼠肺中TNF-α、IL-6、TGF-β、CK-18mRNA和蛋白表达比对照组显著增加。这说明PM_(2.5)亚慢性染毒可增加炎症因子表达水平,促进肺炎症反应。CK-18表达上调提示PM_(2.5)致肺组织处于氧化应激状态。太原市冬季PM_(2.5)引起大鼠肺炎症损伤效应比夏季严重。PM2.5 samples were sampled and PM2.5 saline suspension was prepared,the male SD rats were in- jected with the suspension. The rats were administered using PM2.5 solutions by intratracheal instillation respectively every three days enduring for 60 days. The histopathological changes and the mRNA and pro- tein expressions of inflammatory factors and CK-18 in lung of rats were detected. The results showed that with the PM2.5 dosage increasing,the lung histopathological damage was aggravated accompanied by some inflammatory responses like hyperemia and inflammatory cell infiltrate. Such the effects induced by winter PM2. 5 were significant compared to summer PM2.5. The mRNA and protein expressions of TNF-α, IL-6, TGF-β and CK-18 in the lung tissues in medium and high dose PM2.5 groups in winter were significantly in- creased compared with the control group, while the expressions of the above 4 genes were significantly ele- vated in the high dose PM2.5 group in summer compared to the control. It suggests that subchronic expo- sure of PM2.5 induces an increase of levels of inflammatory related factors, which may enhance the inflam- matory responses in lung. Up-regulation of CK-18 expression suggests that the lung is in the status of oxi- dative stress induced by PM2.5. The inflammatory responses in the lungs of rats caused by winter PM2.5 in Taiyuan were more severe than that of summer PM2.5.

关 键 词：细颗粒物 大鼠 肺 炎症因子 细胞角蛋白18 亚慢性染毒 

分 类 号：R994.6[医药卫生—毒理学]