新疆狗牙根种质芽期耐盐性综合评价  

Evaluation of the salt resistance of the Cynodon dactylon germplasm from Xinjiang during the seed germination period

作　　者：柴艳[1] 孙宗玖[1,2] 李培英[1,2] 巴德木其其格 张向向[1] 杨静[1] 

机构地区：[1]新疆农业大学草业与环境科学学院,新疆乌鲁木齐830052 [2]新疆草地资源与生态重点实验室,新疆乌鲁木齐830052

出　　处：《草业学报》2017年第8期154-167,共14页Acta Prataculturae Sinica

基　　金：国家自然科学基金项目(31160477);新疆维吾尔自治区自然科学基金项目(2014211A027)资助

摘　　要：为了解不同狗牙根种质在芽期耐盐差异程度,采用常规纸上萌发法,研究不同NaCl浓度(0、0.4%、0.8%、1.2%、1.6%)对来源于新疆不同生境下50份狗牙根种质相对发芽率、相对发芽势、发芽指数、胚根长、胚芽长的影响,并通过聚类分析法对其耐盐性进行综合评价。结果表明,随盐胁迫增加,狗牙根种质相对发芽率、相对发芽势、发芽指数呈明显降低趋势,而胚根长、胚芽长则呈先升后降趋势,0.4%盐胁迫下达到最高;各测试指标变异系数呈逐渐加大趋势,至1.6%盐胁迫时其变异系数均较对照增加2.0倍以上。新疆狗牙根半致死NaCl浓度(S50%)均值为1.28%,变异系数为45.66%,且1.2%NaCl可作为其耐盐鉴定最适浓度。聚类分析综合表明,50份新疆狗牙根种质中表现较稳定的耐盐种质为Cd012,中度耐盐种质为Cd019、Cd021、Cd020、Cd054,敏盐种质为Cd001、Cd033、Cd035、Cd057、Cd059。The aim of these experiments was to determine the differences in salt resistance among Cynodon dac- tylon germplasm collected from Xinjiang. The relative germination rate, relative germination energy, germina-tion index, and the length of the radical and plumule of 50 C. dactylon accessions were measured with the pa-per germination method under 0 （control） , 0.4%, 0.8%, 1.2%, and 1.6% NaCl stress. Then, a synthetic e-valuation of salt-resistance was conducted using the cluster analysis method. As the salt concentration in-creased, the relative germination rate, relative germination energy, and germination index of C. dactylon sig-nificantly decreased, and the length of the radical and plumule first increased then decreased, and peaked at 0.4% NaCl. As the salt concentration increased, the variation coefficient of all indexes increased. The variation coefficient under 1. 6% salt stress was more than 2. 0 times that in the control （no NaCl）. The semi-lethal NaCl concentration for the C. dactylon germplasm was 1. 28% （variation coefficient, 45.66%） and 1.2% NaCl was the optimum concentration to evaluate the salt tolerance of C. dactylon. The comprehensive cluster analy-sis of 50 Xinjiang C. dactylon accessions identified Cd012 as the most salt-tolerant accession; Cd019, Cd021, Cd020, and Cd054 as moderately salt-tolerant accessions; and CdOOl, Cd033, Cd035 , Cd057, and Cd059 assalt-sensitive accessions.

关 键 词：狗牙根 耐盐性 芽期 新疆 聚类分析 

分 类 号：S688.4[农业科学—观赏园艺]